| Project/Area Number |
26870283
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
Biological pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | m6A / circadian / m6A methylation / phosphoproteomics / RNA methylation / Circadian clock |
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| Outline of Final Research Achievements |
This proposal had two parts: 1, investigate circadian variations in the m6A methylation of messenger RNA; 2, investigate the roles of Fto, Alkbh5 and Mettl3 in the regulation of gene expression.
Our results for the first part have yielded significant insights on the link between the circadian clock and RNA methylation. We have identified transcripts that are highly methylated across the circadian cycles and that are currently investigated. Moreover, we have identified a significant group of transcripts whose methylation show circadian rhythms of m6A methylation, and are currently preparing a manuscript.For the second part, both Fto and Alkbh5 knock-out mice gave negative results, and no circadian phenotypes were detected. Therefore, we are currently breeding double knock-out mice. Mettl3 knock-out mice were embryonic lethal and conditional knock-out mice have been generated and are currently being mated with SCN-CRE and Alb-CRE to generate SCN and liver-specific KO mice.
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