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Role of RNA methylation in the regulation of gene expression.

Research Project

Project/Area Number 26870283
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental physiology(including physical medicine and nutritional physiology)
Biological pharmacy
Research InstitutionKyoto University

Principal Investigator

Fustin Jean-Michel  京都大学, 薬学研究科(研究院), 講師 (50711818)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsm6A / circadian / m6A methylation / phosphoproteomics / RNA methylation / Circadian clock
Outline of Final Research Achievements

This proposal had two parts: 1, investigate circadian variations in the m6A methylation of messenger RNA; 2, investigate the roles of Fto, Alkbh5 and Mettl3 in the regulation of gene expression.
Our results for the first part have yielded significant insights on the link between the circadian clock and RNA methylation. We have identified transcripts that are highly methylated across the circadian cycles and that are currently investigated. Moreover, we have identified a significant group of transcripts whose methylation show circadian rhythms of m6A methylation, and are currently preparing a manuscript.For the second part, both Fto and Alkbh5 knock-out mice gave negative results, and no circadian phenotypes were detected. Therefore, we are currently breeding double knock-out mice. Mettl3 knock-out mice were embryonic lethal and conditional knock-out mice have been generated and are currently being mated with SCN-CRE and Alb-CRE to generate SCN and liver-specific KO mice.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Invited: 7 results)

  • [Journal Article] Isoform-specific monoclonal antibodies against 3beta-hydroxysteroid dehy drogenase/isomerase family provide markers for subclassification of hum an primary aldosteronism. .2014

    • Author(s)
      Doi M, Satoh F, Maekawa T, N akamura Y, Fustin JM, Tainaka M, Hotta Y, Takahashi Y, Morimoto R, Takase K, Ito S, Sasano H, and Okamura H
    • Journal Title

      J Clin Endocrinol Metab

      Volume: 99 Issue: 2 Pages: 257-62

    • DOI

      10.1210/jc.2013-3279

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] m6A mRNA methylation is coupled to transcription but enhances dynamic changes in mRNA levels2015

    • Author(s)
      Fustin Jean-Michel
    • Organizer
      TGSW2015, Tsukuba, Frontier Science on Transcription and Metabolism,
    • Place of Presentation
      Tsukuba
    • Year and Date
      2015-09-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] What is the most important trait or characteristic to be a successful scientist?2015

    • Author(s)
      Fustin Jean-Michel
    • Organizer
      TGSW2015, Tsukuba, Frontier Science on Transcription and Metabolism,
    • Place of Presentation
      Tukuba
    • Year and Date
      2015-09-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Of RNA methylation and clock genes2015

    • Author(s)
      Fustin Jean-Michel
    • Organizer
      【転写代謝システム】平成27年度班会議, Oral session 4, O-27
    • Place of Presentation
      菊南温泉 ユウベルホテル
    • Year and Date
      2015-06-14
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Role of the epitranscriptome in mammalian circadian system2014

    • Author(s)
      Jean-Michel Fustin
    • Organizer
      MBSJ2014
    • Place of Presentation
      Pacifico Yokohama
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] m6A shows its mettle2014

    • Author(s)
      Jean-Michel Fustin
    • Organizer
      JSC21 第21回日本時間生物学会学術大会
    • Place of Presentation
      大戸 茂弘(九州大学大学院薬学研究院 研究院長・教授)
    • Year and Date
      2014-11-07 – 2014-11-09
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] m6A shows its mettle2014

    • Author(s)
      Jean-Michel Fustin
    • Organizer
      第7回 MSD SYMPHONY
    • Place of Presentation
      ホテルメトロポリタンエドモント飯田橋
    • Year and Date
      2014-09-15
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] m6A shows its mettle2014

    • Author(s)
      Jean-Michel Fustin
    • Organizer
      生命素子による転写環境とエネルギー代謝のクロストーク制御(転写代謝システム)― 班会議 ―
    • Place of Presentation
      仙台 秋保温泉 岩沼屋
    • Year and Date
      2014-07-11 – 2014-07-13
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] m6A shows its mettle2014

    • Author(s)
      Jean-Michel Fustin
    • Organizer
      SRBR
    • Place of Presentation
      Big Sky Resort, Montana
    • Year and Date
      2014-06-14 – 2014-06-18
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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