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Development of fluorescent sensors to quantitatively detect signal molecules in single cells

Research Project

Project/Area Number 26870291
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Chemical biology
Research InstitutionKyoto University

Principal Investigator

Sakaguchi Reiko  京都大学, 物質-細胞統合システム拠点, 助教 (80723197)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsケミカルバイオロジー / 生体関連化学 / シグナル伝達 / タンパク質工学 / 分子認識 / 蛍光イメージング / 単一細胞測定
Outline of Final Research Achievements

Living organism utilize various signaling molecules to regulate themselves. Among them, InsPns are known to regulate intracellular Ca2+ concentrations and control cellular responses such as transcription and translation. Individual InsPns are very similar to each other, with only subtle differences. Nevertheless, they each play their own roles as a signaling molecule. Therefore, it will be useful to develop a technique that can monitor each of the InsPns in a live cell in real time. In this study, we have developed fluorescence based sensors that can specifically monitor several InsPns, to elucidate their physiological roles.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Validating subcellular thermal changes revealed by fluorescent thermosensors2015

    • Author(s)
      Kiyonaka S, Sakaguchi R, Hamachi I, Morii T, Yoshizaki T, Mori Y
    • Journal Title

      Nature Method.

      Volume: 12 Issue: 9 Pages: 801-802

    • DOI

      10.1038/nmeth.3548

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent sensors reveal subcellular thermal changes2015

    • Author(s)
      Reiko Sakaguchi, Shigeki Kiyonaka, Yasuo Mori
    • Journal Title

      Current Opinion in Biotechnology

      Volume: 31 Pages: 57-64

    • DOI

      10.1016/j.copbio.2014.07.013

    • NAID

      120005474467

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Deciphering Subtype-Selective Modulations in TRPA1 Biosensor Channels2014

    • Author(s)
      Daisuke Kozai, Reiko Sakaguchi, Tomohiko Ohwada, Yasuo Mori
    • Journal Title

      Current Neuropharmacology

      Volume: 12

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Identification of the Physiological Role of Inositol (1,3,4,5)-Tetrakisphosphate by Fluorescent Biosensors2015

    • Author(s)
      Reiko Sakaguchi
    • Organizer
      The Second Kitasato-Yale Fluid Symposium: Molecular Control of Cellular and Epithelial Function
    • Place of Presentation
      北里大学(東京都港区)
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 蛍光性センサーを用いたイノシトール四リン酸(IP4)の生理学的意義の探索2015

    • Author(s)
      坂口怜子、森井孝、森泰生
    • Organizer
      生理学研究所 研究会「生体シグナルダイナミクス」
    • Place of Presentation
      生理学研究所(愛知県岡崎市)
    • Year and Date
      2015-09-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells2014

    • Author(s)
      Reiko Sakaguchi
    • Organizer
      The 87th Annual Meeting of the Japanese Biochemical Society
    • Place of Presentation
      Kyoto,Japan
    • Year and Date
      2014-10-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 異なるメチル基転移酵素による転移RNA認識機構の検討2014

    • Author(s)
      坂口 怜子
    • Organizer
      第46回 若手ペプチド夏の勉強会
    • Place of Presentation
      京都府宮津市
    • Year and Date
      2014-08-03
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 京都大学工学研究科森研究室

    • URL

      http://www.sbchem.kyoto-u.ac.jp/mori-lab/member/staff_sakaguchi_reiko.html

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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