Development of fluorescent sensors to quantitatively detect signal molecules in single cells
| Project/Area Number |
26870291
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Bio-related chemistry
Chemical biology
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
Sakaguchi Reiko 京都大学, 物質-細胞統合システム拠点, 助教 (80723197)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | ケミカルバイオロジー / 生体関連化学 / シグナル伝達 / タンパク質工学 / 分子認識 / 蛍光イメージング / 単一細胞測定 |
|---|
| Outline of Final Research Achievements |
Living organism utilize various signaling molecules to regulate themselves. Among them, InsPns are known to regulate intracellular Ca2+ concentrations and control cellular responses such as transcription and translation. Individual InsPns are very similar to each other, with only subtle differences. Nevertheless, they each play their own roles as a signaling molecule. Therefore, it will be useful to develop a technique that can monitor each of the InsPns in a live cell in real time. In this study, we have developed fluorescence based sensors that can specifically monitor several InsPns, to elucidate their physiological roles.
|
Report
(3 results)
Research Products
(8 results)
