The search of novel factors that control the subcellular localization of Lipin1

• Project/Area Number: 26870346
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy; Pathological medical chemistry
• Research Institution: The University of Tokyo (2015); Osaka University (2014)
• Principal Investigator: Ishimoto Kenji 東京大学, 先端科学技術研究センター, 研究員 (00572984)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: Lipin1 / 細胞内光クロスリンク法 / 脂質代謝 / 細胞内局在 / 脂肪酸酸化 / トリグリセリド合成 / 核移行シグナル (NLS) / 肥満 / 核移行シグナル（NLS） / 脂質代謝制御因子 / トリグリセリド生合成 / 免疫沈降法

Outline of Final Research Achievements

We set out to search for novel factors that control the subcellular localization of Lipin1 by using in cell protein photo-cross-linking technique. As a result, we have identified the phosphorylated binding protein as novel Lipin1 interacting factor.

Research Products

• [Journal Article] Analysis of the subcellular localization of the human histone methyltransferase SETDB1 (2015)
  • Author(s): Tachibana K, Gotoh E, Kawamata N, Ishimoto K, Uchihara Y, Iwanari H, Sugiyama A, Kawamura T, Mochizuki Y, Tanaka T, Sakai J, Hamakubo T, Kodama T, Doi T.
  • Journal Title: Biochem Biophys Res Commun Volume: 465 Issue: 4 Pages: 725-731
  • DOI: 10.1016/j.bbrc.2015.08.065
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Expression of the Robo4 receptor in endothelial cells is regulated by two AP-1 protein complexes. (2015)
  • Author(s): Okada Y, Naruse H, Tanaka T, Funahashi N, Regan ER, Yamakawa K, Hino N, Ishimoto K, Doi T, Aird WC.
  • Journal Title: Biochem Biophys Res Commun Volume: 467(4) Issue: 4 Pages: 987-991
  • DOI: 10.1016/j.bbrc.2015.10.029
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Loss of hepatic HIF-1α accelerates lipid accumulation by inhibiting peroxisomal fatty acid oxidation in nonalcoholic fatty liver disease. (2015)
  • Author(s): Arai T, Kato Y, Ishimoto K, Kanai M, Shinjo S, Sayama K, Suzuki T, Doi T, Johnson RS, Suematsu M, Goda N.
  • Journal Title: J Hepatol. Volume: Feb
  • DOI: 10.1016/j.jhep.2015.01.037
  • Peer Reviewed
• [Journal Article] Endothelial cell-specific expression of roundabout 4 is regulated by differential DNA methylation of the proximal promoter (2014)
  • Author(s): Okada, Y., Funahashi, N., Tanaka, T., Nishiyama, Y., Yuan, L., Shirakura, K., Turjman, A. S., Kano, Y., Naruse, H., Suzuki, A., Sakai, M., Zhixia, J., Kitajima, K., Ishimoto, K., Hino, N., Kondoh, M., Mukai, Y., Nakagawa, S., Garcia-Cardena, G., Aird, W. C., Doi, T.
  • Journal Title: Arterioscler Thromb. Vasc. Biol. Volume: 34 Issue: 7 Pages: 1531-1538
  • DOI: 10.1161/atvbaha.114.303818
  • Peer Reviewed
• [Presentation] 脂質代謝制御因子Lipin1と分解メカニズムの解明 (2015)
  • Author(s): 奥野紘子，石本憲司，熊谷文子，早瀬絢香，樋野展正，橘敬祐，川村猛，田中十志也，児玉龍彦，土井 健史
  • Organizer: 第16回 Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2015-06-13
• [Presentation] ユビキチンリガーゼによる脂質代謝調節因子 Lipin1 の分解機構の解析 (2014)
  • Author(s): 早瀬絢香，石本憲司，熊谷文子，橘敬祐，川村猛，田中十志也，浜窪隆雄，酒井寿郎，児玉龍彦，土井健史
  • Organizer: 第64回 日本薬学会近畿支部総会・大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-11