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Investigation of microbial fermentation utilizing membrane separation and electrochemical system

Research Project

Project/Area Number 26870355
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Applied microbiology
Research InstitutionKobe University

Principal Investigator

sasaki kengo  神戸大学, 科学技術イノベーション研究科, 特命准教授 (50558301)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsrice straw / mechanical milling / methane fermentation / ethanol fermentation / nano-filtration / lignin / sweet sorghum juice / ligonocellulosic biomass / supporting material / nano filtration / lignin aromatics / Bioethanol / Mechanical milling / Membrane / Saccharomyces cerevisiae / Rice straw / Sweet sorghum juice
Outline of Final Research Achievements

Utilization of rice straw, which is one of lignocellulosic biomass, was aimed. As the pretreatment, mechanical milling of rice straw increased subsequent production of methane or ethanol by microorganims. (Energenic consumption using mechanical milling was low.)
Liquid fraction of hydrothermally pretreated rice straw was concentrated by nano-filtration (NF) membrane. Enzymatic hydrolysis of this concentrate precipitated lignin easily.
Ultra-filtration permeation and NF concentration were performed on the sweet sorghum juice. Saccharomyces cerevisiae repeatedly produced about 100 g/L of ethanol from the NF concentrate.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (15 results)

All 2016 2015 2014 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 4 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (2 results)

  • [Journal Article] Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae2016

    • Author(s)
      Sasaki K., Hara K.Y., Kawaguchi H., Sazuka T., Ogino C., Kondo A.
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 120 Issue: 1 Pages: 96-100

    • DOI

      10.1016/j.jbiosc.2015.05.013

    • NAID

      110010041309

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content2015

    • Author(s)
      Sasaki, K., Kikuchi, J. (12人中11番目)
    • Journal Title

      Biotech. Biofuels

      Volume: 8 Issue: 1 Pages: 88-88

    • DOI

      10.1186/s13068-015-0273-4

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae2015

    • Author(s)
      Sasaki K, Tsuge Y, Sasaki D, Teramura H, Inokuma K, Hasunuma T, Ogino C, Kondo A
    • Journal Title

      Bioresource Technology

      Volume: 185 Pages: 263-268

    • DOI

      10.1016/j.biortech.2015.02.117

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae2014

    • Author(s)
      Sasaki K, Tsuge Y, Sasaki D, Hasunuma T, Sakamoto T, Sakihama Y, Ogino C, Kondo A
    • Journal Title

      Bioresource Technology

      Volume: 169 Pages: 380-386

    • DOI

      10.1016/j.biortech.2014.06.101

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process2014

    • Author(s)
      Sasaki K, Tsuge Y, Sasaki D, Teramura H, Wakai S, Kawaguchi H, Sazuka T, Ogino C, Kondo A
    • Journal Title

      Bioresource Technology

      Volume: 169 Pages: 821-825

    • DOI

      10.1016/j.biortech.2014.07.082

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 膜分離プロセスによる稲わら水熱処理液からのリグニン回収2016

    • Author(s)
      佐々木建吾・白井智量・柘植陽太・近藤昭彦
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒト大腸フローラモデルを用いたプレバイオティクス効果の検証2016

    • Author(s)
      高木理沙、佐々木建吾、佐々木大介、福田伊津子、大澤朗
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] バイオプロセス高効率化のための遺伝学的・代謝学的解析2016

    • Author(s)
      佐々木建吾
    • Organizer
      第39回広島大学バイオマスイブニングセミナー
    • Place of Presentation
      広島大学(広島県・東広島市)
    • Year and Date
      2016-02-19
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] プレバイオティクス評価系であるヒト大腸フローラモデルのメタゲノム検証2016

    • Author(s)
      高木理沙、佐々木建吾、佐々木大介、福田伊津子、大澤朗
    • Organizer
      日本農芸化学会関西支部例会
    • Place of Presentation
      京都大学楽友会館(京都府・京都市)
    • Year and Date
      2016-02-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Recovery of lignin from rice straw liquid hydrolysate with membrane separation2015

    • Author(s)
      Sasaki K. ・Kondo A.
    • Organizer
      The 6th iBioK Asian Workshop
    • Place of Presentation
      Kobe University(兵庫県・神戸市)
    • Year and Date
      2015-12-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 膜濃縮したソルガム搾汁液からのエタノール発酵2015

    • Author(s)
      佐々木建吾、柘植陽太、佐々木大介、寺村浩、川口秀夫、荻野千秋、春日重光、佐塚隆志、近藤昭彦
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 稲わら前処理液の膜プロセスによるエタノール発酵高効率化2014

    • Author(s)
      佐々木建吾、柘植陽太、佐々木大介、荻野千秋、近藤昭彦
    • Organizer
      日本生物工学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 膜プロセスによるエタノール発酵高効率化2014

    • Author(s)
      佐々木建吾、近藤昭彦
    • Organizer
      第38回九州紙パルプ研究会講演会
    • Place of Presentation
      福岡大学
    • Year and Date
      2014-06-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 神戸大学 大学院科学技術イノベーション研究科 佐々木建吾

    • URL

      http://www2.kobe-u.ac.jp/~akondo/sasaki.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 神戸大学大学院工学研究科応用科学専攻バイオ生産工学研究室

    • URL

      http://www2.kobe-u.ac.jp/~akondo/sasaki.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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