| Project/Area Number |
26870364
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
Human pathology
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| Research Institution | Kobe University |
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Principal Investigator |
Koma Yuichiro 神戸大学, 医学(系)研究科(研究院), 講師 (40714647)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 腫瘍関連マクロファージ / 食道扁平上皮癌 / 食道上皮内腫瘍 / p38 MAPキナーゼ / インターロイキン6 |
|---|
| Outline of Final Research Achievements |
In this study, we established coculture assay system using immortalized human esophageal epithelial cells (Het-1A) and human acute monocytic leukemia cells (THP-1)-derived macrophages to study the roles of macrophages in esophageal carcinogenesis. Coculture of Het-1A cells with THP-1-derived macrophages induced Het-1A cell proliferation/migration and phosphorylation of p38 MAP kinase. In addition, IL-6 was significantly increased in coculture supernatants. Finally, positive immunoreactivity for phospho-p38 MAP kinase and IL-6 in neoplastic cells were observed in some cases of esophageal high grade intraepithelial neoplasias.
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