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A filamentous phage affects host pathogenicity according to phage infection stage.

Research Project

Project/Area Number 26870399
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant protection science
Applied microbiology
Research InstitutionHiroshima University

Principal Investigator

Kawasaki Takeru  広島大学, 先端物質科学研究科, 助教 (00510299)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords分子生物学 / 植物病原菌 / 青枯病菌 / ファージ / 植物病理学 / 微生物
Outline of Final Research Achievements

Ralstonia solanacearum is a causative agent of lethal bacterial wilt more than 200 species in 50 botanical families, including economically important crops. R. solanacearum is easily spread via contaminated irrigation water and can survive for many years in the soil.
Recently, there are some reports of host behavioral change caused by lysogenic phage infection. In the case of R. solanacearum, we reported a filamentous phage RSS1 infection increase the host pathogenicity. So we tried to research host pathogenicity change according to phage infection stage, lysogenic cycle and active propagate stage.
And we also tried to find the integration/induction conditions of RSS1 phage. We succeeded to detect inducted RSS1 phage under freeze-thaw condition and RSL1 infected condition.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 Other

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 4 results,  Open Access: 2 results) Presentation (1 results) Remarks (3 results)

  • [Journal Article] The involvement of the PilQ secretin of type IV pili in phage infection in Ralstonia solanacearum2016

    • Author(s)
      Narulita, E., H. S. Addy, T. Kawasaki, M. Fujie and T. Yamada.
    • Journal Title

      Biochem. Biophys. Res. Comm.

      Volume: 469 Issue: 4 Pages: 868-872

    • DOI

      10.1016/j.bbrc.2015.12.071

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genomic diversityoflarge-plaque-formingpodovirusesinfectingthe phytopathogen Ralstoniasolanacearum2016

    • Author(s)
      Takeru Kawasaki 他10名
    • Journal Title

      Virology

      Volume: 492 Pages: 73-81

    • DOI

      10.1016/j.virol.2016.02.011

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Isolation of Ralstonia solanacearum-infecting bacteriophages from tomato fields in Chiang Mai, Thailand, and their experimental use as biocontrol agents2015

    • Author(s)
      Anjana Bhunchoth 他8名
    • Journal Title

      J. Appl. Microbiol.

      Volume: 118 Issue: 4 Pages: 1023-1033

    • DOI

      10.1111/jam.12763

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Identification of the mcpA and mcpM genes, encoding methyl-accepting proteins involved in amino acid and l-malate chemotaxis, and involvement of McpM-mediated chemotaxis in plant infection by Ralstonia pseudosolanacearum (formerly Ralstonia solanacearum phylotypes I and III)2015

    • Author(s)
      A. Hida, S. Oku, T. Kawasaki, Y. Nakashimada, T. Tajima, J. Kato
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 81 Issue: 21 Pages: 7420-7430

    • DOI

      10.1128/aem.01870-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Insights into the diversity of φRSM phages infecting strains of the phytopathogen Ralstonia solanacearum complex : regulation and evolution2014

    • Author(s)
      Ahmed Askora, Takeru Kawasaki, Makoto Fujie and Takashi Yamada
    • Journal Title

      Mol. Genet. Genom.

      Volume: 289(in press) Issue: 4 Pages: 589-598

    • DOI

      10.1007/s00438-014-0835-3

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The filamentous phage XacF1 causes loss of virulence in Xanthomonas axonopodis pv. citri, the causative agent of citrus canker disease.2014

    • Author(s)
      Ahmad, A.A., Askora, A., Kawasaki,T., Fujie, M., and Yamada,T.
    • Journal Title

      Front. Microbiol.

      Volume: 5 Pages: 3211-11

    • DOI

      10.3389/fmicb.2014.00321

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 宿主病原性に影響を与える溶原性繊維状ファージRSSの溶原/誘発機構の解析2015

    • Author(s)
      川崎 健
    • Organizer
      第67回 日本生物工学会大会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Remarks] 広島大学大学院 先端研 先端分子情報学研究室(山田研) 一講ホームページへようこそ!

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 公表論文

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/pg46.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 広島大学大学院 先端研 生命分子情報学研究室(山田研) 一講ホームページにようこそ!

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/index.html

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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