Developing a new preventive approach of foodborne diseases caused by VBNC/injured bacteria using Light-Emitting Diode irradiation

• Project/Area Number: 26870411
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits; Applied health science
• Research Institution: The University of Tokushima
• Principal Investigator: AIHARA Mutsumi 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 徳島大学専門研究員 (60596211)
• Project Period (FY): 2014-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 食中毒予防 / VBNC / UV-LED / 食中毒 / 腸炎ビブリオ / 紫外線LED / DNAマイクロアレイ / マイクロアレイ / LED / 損傷菌 / 食品

Outline of Final Research Achievements

VBNC (Viable But Non-Culturable) state bacteria poses potential health risks especially foodborne disease because it includes ability of recovery or resuscitation in the body. Injured bacteria is referred to as a state of
VBNC or near VBNC state during food preservation or sterilization. VBNC state is induced by many factors such as temperature downshift, pH change, nutrient condition or oxygen concentration. However VBNC/injured bacteria occurrence and mechanism of resuscitation are not yet understood well. In this study, in order to develop a new preventive approach of foodborne diseases, level of gene expression was compared on our hypothesis that specific wavelength of light would be a factor of VBNC induction or resuscitation.

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的基盤となる「光による病原微生物の応答と制御」の分子機構は光の受容体の同定を含めてを未だ解明されていない点が多いが、本研究課題で取り入れた網羅的な遺伝子発現に加えて代謝機能解析を合わせたことでVBNC・損傷菌の発生や蘇生と修復を制御する重要な知見が得られた。
現在我が国における食品衛生管理は、塩素系薬剤など薬剤を使用した方法が主流のため、光による衛生管理システムは欧州に 比べると立後れているといえる。本研究の成果は、分子メカニズムのエビデンスを基盤とした光による損傷菌・VBNC 菌に対する食中毒感染予防への応用可能性が示唆され、食品衛生管理の質の向上が期待できる。

Research Products

• [Journal Article] Irradiation by ultraviolet light-emitting diodes inactivates influenza a viruses by inhibiting replication and transcription of viral RNA in host cells (2018)
  • Author(s): Nishisaka-Nonaka Risa、Mawatari Kazuaki、Yamamoto Tomomi、Kojima Mizuki、Shimohata Takaaki、Uebanso Takashi、Nakahashi Mutsumi、Emoto Takahiro、Akutagawa Masatake、Kinouchi Yohsuke、Wada Takahiro、Okamoto Masayuki、Ito Hiroshi、Yoshida Ken-ichi、Daidoji Tomo、Nakaya Takaaki、Takahashi Akira
  • Journal Title: Journal of Photochemistry and Photobiology B: Biology Volume: 189 Pages: 193-200
  • DOI: 10.1016/j.jphotobiol.2018.10.017
  • Peer Reviewed / Open Access
• [Journal Article] UVA‐LED device to disinfect hydroponic nutrient solution (2018)
  • Author(s): Tsunedomi Akari、Miyawaki Katsuyuki、Masamura Akinori、Nakahashi Mutsumi、Mawatari Kazuaki、Shimohata Takaaki、Uebanso Takashi、Kinouchi Yousuke、Akutagawa Masatake、Emoto Takahiro、Takahashi Akira
  • Journal Title: The Journal of Medical Investigation Volume: 65 Issue: 3.4 Pages: 171-176
  • DOI: 10.2152/jmi.65.171
  • NAID: 130007495838
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed / Open Access
• [Journal Article] Cellular Tight Junctions Prevent Effective Campylobacter jejuni Invasion and Inflammatory Barrier Disruption Promoting Bacterial Invasion from Lateral Membrane in Polarized Intestinal Epithelial Cells (2018)
  • Author(s): Hatayama Sho、Shimohata Takaaki、Amano Sachie、Kido Junko、Nguyen Anh Q.、Sato Yuri、Kanda Yuna、Tentaku Aya、Fukushima Shiho、Nakahashi Mutsumi、Uebanso Takashi、Mawatari Kazuaki、Takahashi Akira
  • Journal Title: Frontiers in Cellular and Infection Microbiology Volume: 8
  • DOI: 10.3389/fcimb.2018.00015
  • Peer Reviewed / Open Access
• [Journal Article] Effects of Low-Dose Non-Caloric Sweetener Consumption on Gut Microbiota in Mice (2017)
  • Author(s): Uebanso Takashi、Ohnishi Ai、Kitayama Reiko、Yoshimoto Ayumi、Nakahashi Mutsumi、Shimohata Takaaki、Mawatari Kazuaki、Takahashi Akira
  • Journal Title: Nutrients Volume: 9 Issue: 6 Pages: 560-560
  • DOI: 10.3390/nu9060560
  • Peer Reviewed / Open Access
• [Journal Article] Cystic Fibrosis Transmembrane Conductance Regulator Reduces Microtubule-Dependent Campylobacter jejuni Invasion (2017)
  • Author(s): Kido Junko、Shimohata Takaaki、Amano Sachie、Hatayama Sho、Nguyen Anh Quoc、Sato Yuri、Kanda Yuna、Tentaku Aya、Fukushima Shiho、Nakahashi Mutsumi、Uebanso Takashi、Mawatari Kazuaki、Takahashi Akira
  • Journal Title: Infection and Immunity Volume: 85 Issue: 10
  • DOI: 10.1128/iai.00311-17
  • Peer Reviewed / Open Access
• [Journal Article] 紫外線LEDの医療への応用 (2016)
  • Author(s): 中橋睦美,高橋章
  • Journal Title: 小児科 Volume: 57 Pages: 1011-1016
  • Peer Reviewed
• [Journal Article] 紫外線LEDを用いた殺菌システムと応用 (2016)
  • Author(s): 中橋睦美,高橋章
  • Journal Title: ケミカルエンジニヤリング Volume: 61 Pages: 428-432
• [Journal Article] DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus. (2015)
  • Author(s): Quang Ngoc Phan, Takashi Uebansou, Takaaki Shimohata, Mutsumi Nakahashi, Kazuaki Mawatari and Akira Takahashi
  • Journal Title: Journal of Bacteriology Volume: 197 Issue: 18 Pages: 2958-2964
  • DOI: 10.1128/jb.00306-15
  • Peer Reviewed / Open Access
• [Journal Article] Effects of UV-A LED light irradiation on growth of cultured RAW 264.7 cells (2015)
  • Author(s): Toshitaka Ikehara, Mutsumi Nakahashi, Zehong Su, Masatake Akutagawa, Koichiro Tsuchiya, Mitsuo Kitamura, Akira Takahashi and Yohsuke Kinouchi
  • Journal Title: Toxicological and Environmental Chemistry Volume: 97 Issue: 2 Pages: 243-255
  • DOI: 10.1080/02772248.2015.1039771
  • Peer Reviewed / Open Access
• [Journal Article] Simultaneous irradiation with different wavelengths of ultraviolet light has synergistic bactericidal effect on Vibrio parahaemolyticus (2014)
  • Author(s): Mutsumi Nakahashi, Kazuaki Mawatari, Akiko Hirata, Miki Maetani, Takaaki Shimohata, Takashi Uebansou, Yasuhiro Hamada, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi
  • Journal Title: Photochemistry and Photobiology Volume: 90 Issue: 8 Pages: 1397-1403
  • DOI: 10.1088/0967-3334/34/8/925
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Developing a new preventive approach of foodborne disease caused by VBNC/injured bacteria using Xenon lamp and Light-Emitting Diode irradiation (2019)
  • Author(s): Mutsumi Nakahashi, Takaaki Shimohata, Takashi Uebanso, Kazuaki Mawatari, Akira Takahashi
  • Organizer: U.S.-Japan Cooperative Medical Sciences Program (USJCMSP) 53rd Joint US-Japan Panel Meeting on Cholera and Other Bacterial Enteric Infections
  • Int'l Joint Research
• [Presentation] CFTR reduced microtubule-mediated Campylobacter jejuni invasion (2017)
  • Author(s): 木戸 純子, 下畑 隆明, 天野 幸恵, 畑山 翔, 佐藤 優里, 神田 結奈, 天宅 あや, 福島 志帆, 中橋 睦美, 上番増 喬, 馬渡 一諭, 髙橋 章
  • Organizer: 日米医学協力研究会コレラ・細菌性腸管感染症専門部会
  • Int'l Joint Research
• [Presentation] 許容上限量の甘味料の摂取が腸内細菌叢と宿主へ及ぼす影響の解析 (2017)
  • Author(s): 上番増 喬, 大西 愛, 北山 礼子, 吉本 亜由美, 中橋 睦美, 下畑 隆明, 馬渡 一諭, 髙橋 章
  • Organizer: 日本栄養食糧学会
• [Presentation] Campylobacter jejuni食中毒予防に対するUVA-LED照射殺菌の有効性について (2016)
  • Author(s): 常冨愛香里，下畑隆明，永田早紀恵，天野幸恵，中橋睦美，原田優美，上番増喬，馬渡一諭，宮脇克行，榎本崇宏，芥川正武，木内陽介，高橋 章
  • Organizer: 第37回日本食品微生物学会学術総会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2016-09-15
• [Presentation] 飲料水の色が近紫外線殺菌に与える影響 (2016)
  • Author(s): 高橋 章，下畑隆明，馬渡一諭，上番増喬，常冨愛香里，中橋睦美，芥川正武，木内陽介
  • Organizer: 第37回日本食品微生物学会学術総会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2016-09-15
• [Presentation] 紫外線LEDと次亜塩素酸ナトリウムの併用による食品殺菌装置の開発 (2016)
  • Author(s): 中橋睦美、常冨愛香里、上番増喬、下畑隆明、馬渡一諭、芥川正武、木内陽介、高橋 章
  • Organizer: 第37回日本食品微生物学会学術総会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2016-09-15
• [Presentation] UVA-LED殺菌システムによるCampylobacter jejuni食中毒の予防 (2016)
  • Author(s): 常冨 愛香里, 下畑 隆明, 後藤 茉凜, 天野 幸恵, 中橋 睦美, 原田 優美, 上番増 喬, 馬渡 一諭, 宮脇 克行, 榎本 崇宏, 芥川 正武, 木内 陽介, 髙橋 章
  • Organizer: 第70回日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学(兵庫県西宮市)
  • Year and Date: 2016-05-13
• [Presentation] 5.Disinfection system with UVA-LED for hydroponic nutrient solution (2015)
  • Author(s): Akari Tsunedomi, Akinori Masamura, Mutsumi Nakahashi, Risa Nishisaka, Kazuaki Mawatari，Takaaki Shimohata, Takashi Uebanso, Katsuyuki Miyawaki, Masatake Akutagawa, Yousuke Kinouchi, Akira Takahashi
  • Organizer: FEMS2015
  • Place of Presentation: オランダ・マーストリヒト
  • Year and Date: 2015-06-07
  • Int'l Joint Research
• [Presentation] 6.The denudation of lateral part promote Campylobacter jejuni invasion in Caco-2 cells. (2015)
  • Author(s): Sho Hatayama, Takaaki Shimohata, Sachie Negoro, Yuri Sato, Junko Kido, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari, Akira Takahashi
  • Organizer: FEMS2015
  • Place of Presentation: オランダ・マーストリヒト
  • Year and Date: 2015-06-07
  • Int'l Joint Research
• [Presentation] 7.INFECTION OF CAMPYLOBACTER JEJUNI REDUCES CFTR MEDIATED CL- SECRETION IN T-84 (2015)
  • Author(s): Takaaki Shimohata, Sachie Negoro1, Junko Kido, Sho Hatayama, Yuri Sato, Hitomi iba, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari, Akira Takahashi
  • Organizer: FEMS2015
  • Place of Presentation: オランダ・マーストリヒト
  • Year and Date: 2015-06-07
  • Int'l Joint Research
• [Presentation] 8.COMBINATION BETWEEN A FEW T3SS INJECTISOME AND A LOT EFFECTOR FOR KILLING HOST CELLS ON VIBRIO PARAHAEMOLYTICUS (2015)
  • Author(s): Takashi Uebanso, Takaaki Shimohata, Hitomi Iba, Kazuya Nishimura, Yuichi Taniguchi, Kazuki Horikawa, Mutsumi Aihara, Kazuaki Mawatari, Akira Takahashi
  • Organizer: FEMS2015
  • Place of Presentation: オランダ・マーストリヒト
  • Year and Date: 2015-06-07
  • Int'l Joint Research
• [Presentation] DNA-biding protein HU coordiantes pathogenicity in Vibrio parahaemolyticus (2015)
  • Author(s): Phan Ngoc Quang；上番増 喬；馬渡 一諭；下畑 隆明；中橋 睦美；高橋 章
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場(岐阜県岐阜市)
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 腸炎ビブリオの VP2357 遺伝子変異は近紫外線（UVA）照射耐性 獲得に寄与する (2015)
  • Author(s): 馬渡 一諭；本庄 アイリ；安井 実希；畑山 翔；中橋 睦美； 下畑 隆明；高橋 章
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場(岐阜県岐阜市)
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Campylobacter jejuni の侵入性は CFTR によって制御を受ける (2015)
  • Author(s): 下畑 隆明；木戸 純子；根来 幸恵；畑山 翔；佐藤 優里；中 橋 睦美；上番増 喬；馬渡 一諭；高橋 章
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場(岐阜県岐阜市)
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Campylobacter jejuni は腸管上皮の側部より効率的に侵入する (2015)
  • Author(s): 畑山 翔；下畑 隆明；根来 幸恵；佐藤 優里；木戸 純子；中 橋 睦美；上番増 喬；馬渡 一諭；高橋 章
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場(岐阜県岐阜市)
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 近紫外線による水耕栽培用培養液の殺菌法の開発 (2014)
  • Author(s): 常冨愛香里；正村彰規；粟飯原睦美；馬渡一諭；下畑隆明；上番増喬；石崎仁愛；芥川正武；木内陽介；高橋章
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 酪農学園大学(北海道江別市)
  • Year and Date: 2014-05-30 – 2014-06-01
• [Presentation] Vibrio parahaemolyticus のkatG gene,VPA0768 発現上昇は近紫外線（UVA）耐性獲得に関与する (2014)
  • Author(s): 本庄アイリ；馬渡一諭；前谷美希；常冨愛香里；畑山翔；粟飯原睦美；下畑隆明；上番増喬；高橋章
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 酪農学園大学(北海道江別市)
  • Year and Date: 2014-05-30 – 2014-06-01