The development of endogenous RNA imaging technique
| Project/Area Number |
26870430
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanobioscience
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
Yagi Yusuke 九州大学, (連合)農学研究科(研究院), 研究員 (60612421)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | RNA / タンパク質工学 / 一分子イメージング |
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| Outline of Final Research Achievements |
In this study, we had tried to develop new RNA imaging tool using the PPR motif that binds to RNA sequence-specific manner. The basic method for constructing custom PPR protein that binds to any RNA sequence was established. The RNA binding performance of the constructed custom PPR was found to have sufficient performance to function in cultured animal cell. Furthermore, we have established a method for stable expressing GFP fused PPR protein in animal cultured cell. Thus, we were able to build a basic part for PPR-RNA imaging system.
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Report
(3 results)
Research Products
(7 results)
