| Project/Area Number |
26870440
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
Risk sciences of radiation and chemicals
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | DNA二重鎖切断 / 抗酸化剤 / comet assay / 放射線 / 内部被ばく / 甲状腺 / 発癌 / DNA損傷 |
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| Outline of Final Research Achievements |
To establish the in vitro model of the external irradiation with X-ray or internal irradiation with 131-I for analysis of radiation-induced DNA damage response, DNA double strand breaks (DSBs), as an index for DNA damages, were quantified following external or internal irradiation using the neutral comet assay in a normal rat thyroid cell line PCCL3. Then by using this new model established, the inhibitory effect of an anti-oxidant NAC on DNA damages was evaluated. In internal irradiation, administration of NAC even after addition of 131-I was effective at suppressing DSBs. However, this type of in vivo model was failed to be established, and mouse model of radiation-induced thyroid cancer using genetically modified mice showed no histological alterations.
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