| Project/Area Number |
26870442
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
Genetics/Chromosome dynamics
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| Research Institution | Kumamoto University |
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Principal Investigator |
Ideue Takashi 熊本大学, 自然科学研究科, 助教 (20420250)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ノンコーディングRNA / セントロメア / 染色体分離 / RNP / 抗がん戦略 |
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| Outline of Final Research Achievements |
Non-coding (nc)RNA transcribed from human centromere region forms RNA-Protein complex with several factors, and have a role in chromosome segregation and cytokinesis. In this study, I have identified several factors associate with centromere ncRNA and analyzed these functions in chromosome segregation and cytokinesis as a components of centromere ncRNP.
RBMX, IMP-3, IQGAP1 are identified as a components of centromeric ncRNP. IP experiment revealed that these factors associates with centromere ncRNA. Depletion of RBMX showed abnormal chromosome segregation and sister chromatid separation.IMP-3, IQGAP1 and Centromere ncRNA shows localization of central spindle and midbody which concern to cytokinesis after chromosome segregation. Depletion of ncRNA and these factors show abnormal cytokinesis in addition of defects of chromosome segregation.These results reveals that these factors have a role in not only chromosome segregation but also cytokinesis together with centromeric ncRNA.
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