CCL8 KOマウスにおけるGVHD消失の分子機構解明に向けた基礎的研究

• Project/Area Number: 26870464
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology; Pediatrics
• Research Institution: Sapporo Medical University
• Principal Investigator: 武井 則雄 札幌医科大学, 医学部, 助教 (50523461)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Discontinued (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ＧＶＨＤ / CCL8 / ZFN / ケモカイン / KOマウス / 移植片対宿主病

Outline of Annual Research Achievements

前年度の研究結果を踏まえ、KOマウスのGVHD発症機構と関連の予想される主要組織適合抗原複合体(MHC)の発現等を含めたphenotype解析の追加実験および、in vitroでのallo recognitionにおけるサイトカイン産生などをWTとKOマウスで比較検証することでKOマウスを用いたGVHDマウスモデル解析におけるGVHD病態変化の要因を探索した。前年度からの本研究成果をまとめ現在論文投稿準備中である。

Research Products

• [Journal Article] Cytoplasmic Fragment of Alcadein α Generated by Regulated Intramembrane Proteolysis Enhances Amyloid β-Protein Precursor (APP) Transport into the Late Secretory Pathway and Facilitates APP Cleavage (2015)
  • Author(s): Norio Takei, Yuriko Sobu, Ayano Kimura, Satomi Urano, Yi Piao, Yoichi Araki, Hidenori Taru, Tohru Yamamoto, Saori Hata, Tadashi Nakaya, Toshiharu Suzuki
  • Journal Title: Journal of Biological Chemistry Volume: 290 Issue: 2 Pages: 987-995
  • DOI: 10.1074/jbc.m114.599852
  • Peer Reviewed / Open Access
• [Journal Article] Deficiency of NOX1/nicotinamide adenine dinucleotide phosphate, reduced form oxidase leads to pulmonary vascular remodeling. (2014)
  • Author(s): Iwata K, Ikami K, Matsuno K, Yamashita T, Shiba D, Ibi M, Matsumoto M, Katsuyama M, Cui W, Zhang J, Zhu K, Takei N, Kokai Y, Ohneda O, Yokoyama T, Yabe-Nishimura C.
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 34(1) Issue: 1 Pages: 110-119
  • DOI: 10.1161/atvbaha.113.302107
  • Peer Reviewed
• [Presentation] CCL8 Deficiency in Host Abolishes Early Mortality in Murine Graft-Versus-Host Disease Model (2014)
  • Author(s): Igarashi K, Takei N, Hori T, Yamamoto M, Kokai Y.
  • Organizer: 56th ASH Annual Meeting and Exposition
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2014-12-06 – 2014-12-09