New therapeutic strategy for rheumatoid arthritis by controlling cell-cell interactions

• Project/Area Number: 26870493
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology; Orthopaedic surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TSUCHIDA SHINJI 京都府立医科大学, 医学(系)研究科(研究院), 助教 (10719834)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細胞間結合装置 / ギャップ結合 / コネキシン / 関節リウマチ / 滑膜炎 / connexin 43

Outline of Final Research Achievements

The expression of connexin 43 (Cx43) in synovial tissue from eight patients with RA , five patients with osteoarthritis, and one normal female subject was analyzed by quantitative reverse transcriptase polymerase chain reaction and immunohistochemistry of tissue sections. This study shows that Cx43 is expressed in RA synovial tissue and that its expression is induced by stimulation with TNF-a. The expression of the pro-inflammatory cytokines was inhibited by transfection of siCx43. Cx43 may be a novel marker of inflammation in RA synovial tissue.

Research Products

• [Journal Article] Expression of connexin 43 in synovial tissue of patients with rheumatoid arthritis. (2016)
  • Author(s): Matsuki T, Arai Y, Tsuchida S, Terauchi R, Oda R, Fujiwara H, Mazda O, Kubo T.
  • Journal Title: Arch Rheumatol Volume: 31 Issue: 1 Pages: 1-4
  • DOI: 10.5606/archrheumatol.2016.5597
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Analysis of the expression of connexin 43 in synovial tissue of patients with arthritis (2017)
  • Author(s): Seiji Shimomura, Shinji Tsuchida, Yuji Arai, Shuji Nakagawa, Hiroaki Inoue, Shohei Ichimaru, Osam Mazda, Toshikazu Kubo
  • Organizer: Orthopaedic Research Society 2017 Annual Meeting
  • Place of Presentation: Sandiego Convention Center , CA, USA
  • Year and Date: 2017-03-20
  • Int'l Joint Research
• [Presentation] Analysis of the expression of connexin 43 in synovial tissue of patients with arthritis (2016)
  • Author(s): Shimomura S, Tsuchida S, Arai Y, Nakagawa S, Inoue H, Ichimaru S, Mazda O, KuboT
  • Organizer: 19th World Congress of the Osteoarthritis Research Society International (OARSI)
  • Place of Presentation: アムステルダム（オランダ）
  • Year and Date: 2016-03-31
  • Int'l Joint Research
• [Presentation] The roles of connexin 43 in synovitis (2014)
  • Author(s): Tsuchida S, Arai Y, Terauchi R, Nakagawa S, Matsuki T, Ichimaru S, Fujiwara H, Oda R, Kubo T.
  • Organizer: The 24th Japanese-Korean Combined Orthopaedic Symposium
  • Place of Presentation: Hakone, Japan
  • Year and Date: 2014-06-05 – 2014-06-07
• [Presentation] The roles of connexin 43 in experimental arthritis (2014)
  • Author(s): Tsuchida S, Arai Y, Nakagawa S, Terauchi R, Honjo K, Inoue H, Matsuki T, Tokunaga D, Mazda O, Kubo T.
  • Organizer: 17th World Congress of the Osteoarthritis Research Society International (OARSI)
  • Place of Presentation: Paris, France
  • Year and Date: 2014-04-24 – 2014-04-27
• [Presentation] ヒト関節リウマチ滑膜におけるConnexin 43の発現 (2014)
  • Author(s): 松木智宏，新井祐志，土田真嗣，本城邦晃，中川周士，井上裕章，寺内 竜，井上敦夫，市丸昌平，谷口大吾，小田 良，藤原浩芳，徳永大作，川人 豊，久保俊一
  • Organizer: 第58回日本リウマチ学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-24 – 2014-04-26