| Project/Area Number |
26870504
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
Biofunction/Bioprocess
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
YAMADA Ryosuke 大阪府立大学, 工学(系)研究科(研究院), 助教 (40608626)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 酵母 / バイオディーゼル燃料 / リパーゼ / 細胞表層提示 / 遺伝子組換え / 薬剤耐性 |
|---|
| Outline of Final Research Achievements |
Dwindling petroleum supplies and growing environmental concerns over petroleum use have led to increasing interest in using biodiesel fuel for transportation. In this study, we aimed to construct novel biodiesel production strategy comprised lipid production by oleaginous yeast and esterification of produced lipid by lipase expressed on yeast cell surface without addition of lipase. As a result, lipid production was improved by UV-mutagenesis, cell surface lipase activity was improved by combinatorial library strategy, and biodiesel fuel was successfully produced by lipase displayed on yeast cell surface.
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