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Exploratory analyses of comprehensive and quantitative mutation profiling using cell-free DNA

Research Project

Project/Area Number 26870509
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor therapeutics
Tumor diagnostics
Research InstitutionWakayama Medical University

Principal Investigator

Akamatsu Hiroaki  和歌山県立医科大学, 医学部, 助教 (10646582)

Co-Investigator(Renkei-kenkyūsha) KIKUCHI Takashi  和歌山県立医科大学, 内科学第3講座, 助教 (40649050)
YAMAMOTO Nobuyuki  和歌山県立医科大学, 内科学第3講座, 教授 (60298966)
KOH Yasuhiro  和歌山県立医科大学, 内科学第3講座, 講師 (80426519)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsリキッドバイオプシー / EGFR遺伝子変異 / 肺がん / liquid biopsy / Liquid Biopsyに関する研究
Outline of Final Research Achievements

To establish multiplex assay of EGFR mutation using digital PCR, we first develop highly sensitive assay with plasmid and cell-lines. Next, we compared multiplex assay with duplex assay. Sensitivity was highly correlated (R2 = 0.99) between these assays. Thus, we subsequently analyzed clinical samples (60 advanced lung cancer patients and 17 healthy volunteers). Compared with tumor tissues, multiplex assay using plasma samples demonstrated sensitivity of 66% and specificity of 100%, respectively. Of those, patients who had distant metastasis demonstrated higher sensitivity (81%). Thus, this population may be suitable to further clinical trial.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (37 results)

All 2015 2014

All Journal Article (22 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 22 results,  Open Access: 22 results,  Acknowledgement Compliant: 4 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Book (6 results)

  • [Journal Article] Prognostic significance of diabetes mellitus in locally advanced non-small cell lung cancer.2015

    • Author(s)
      Imai H, Kaira K, Mori K, Ono A, Akamatsu H, et al.
    • Journal Title

      BMC Cancer

      Volume: 15 Issue: 1 Pages: 989-989

    • DOI

      10.1186/s12885-015-2012-4

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Progression of Irreversible Airflow Limitation in Asthma: Correlation with Severe Exacerbations2015

    • Author(s)
      Matsunaga K, Hirano T, Oka A, Tanaka A, Kanai K, Kikuchi T, Hayata A, Akamatsu H, Akamatsu K, Koh Y, Nakanishi M, Minakata Y, Yamamoto N
    • Journal Title

      J Allergy Clin Immunol Pract

      Volume: 3 Issue: 5 Pages: 759-764

    • DOI

      10.1016/j.jaip.2015.05.005

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparison of platinum combination re-challenge therapy and docetaxel monotherapy in non-small cell lung cancer patients previously treated with platinum-based chemoradiotherapy.2015

    • Author(s)
      Imai H, Kaira K, Akamatsu H, et al.
    • Journal Title

      Springerplus.

      Volume: 31:4 Issue: 1 Pages: 152-152

    • DOI

      10.1186/s40064-015-0929-3

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The efficacy of amrubicin on central nervous system metastases originating from small-cell lung cancer: a case series of eight patients.2015

    • Author(s)
      Miura S, Kaira K, Akamatsu H, et al.
    • Journal Title

      Invest New Drugs.

      Volume: 33(3) Issue: 3 Pages: 55-60

    • DOI

      10.1007/s10637-015-0233-7

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Disease flare after gefitinib discontinuation.2015

    • Author(s)
      Akamatsu H, Ono A, Shukuya T
    • Journal Title

      Respir Investig.

      Volume: 53(2) Issue: 2 Pages: 68-72

    • DOI

      10.1016/j.resinv.2014.10.005

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Progression-free survival, post-progression survival, and tumor response as surrogate markers for overall survival in patients with extensive small cell lung cancer.2015

    • Author(s)
      Imai H, Mori K, Wakuda K, Ono A, Akamatsu H,
    • Journal Title

      Ann Thorac Med.

      Volume: 10(1) Pages: 61-66

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prognostic impact of cancer cachexia in patients with advanced non-small cell lung cancer.2015

    • Author(s)
      Kimura M, Naito T, Akamatsu H, et al.
    • Journal Title

      Support Care Cancer.

      Volume: 23(6) Issue: 6 Pages: 1699-708

    • DOI

      10.1007/s00520-014-2534-3

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The effect of gefitinib in patients with postoperative recurrent non-small cell lung cancer harboring mutations of the epidermal growth factor receptor.2015

    • Author(s)
      Ko R, Kenmotsu H, Akamatsu H, et al.
    • Journal Title

      Int J Clin Oncol.

      Volume: 20(4) Issue: 4 Pages: 668-73

    • DOI

      10.1007/s10147-014-0761-8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Disease flare after gefitinib discontinuation.2015

    • Author(s)
      Akamatsu H, Ono A, Shukuya T, Tsuya A, Nakamura Y, Kenmotsu H, Naito T, Murakami H, Endo M, Nakajima T, Yamamoto N
    • Journal Title

      Respir Investig

      Volume: 53 Pages: 68-72

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of platinum combination re-challenge therapy and docetaxel monotherapy in non-small cell lung cancer patients previously treated with platinum-based chemoradiotherapy.2015

    • Author(s)
      Imai H, Kaira K, Mori K, Ono A, Akamatsu H, Taira T, Yoshino R, Kenmotsu H, Saitoh J, Harada H, Naito T, Murakami H, Tomizawa Y, Matsuura M, Saito R, Nakajima T, Yamada M, Takahashi T.
    • Journal Title

      Springerplus

      Volume: 4 Pages: 152-152

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The efficacy of amrubicin on central nervous system metastases originating from small-cell lung cancer: a case series of eight patients.2015

    • Author(s)
      Miura S, Kaira K, Kaira R, Akamatsu H, Ono A, Shukuya T, Tsuya A, Nakamura Y, Kenmotsu H, Naito T, Murakami H, Takahashi T, Endo M, Yamamoto N.
    • Journal Title

      Invest New Drugs.

      Volume: epub ahead of print

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Progression-free survival, post-progression survival, and tumor response as surrogate markers for overall survival in patients with extensive small cell lung cancer.2015

    • Author(s)
      Imai H, Mori K, Wakuda K, Ono A, Akamatsu H, Shukuya T, Taira T, Kenmotsu H, Naito T, Kaira K, Murakami H, Endo M, Nakajima T, Yamamoto N, Takahashi T.
    • Journal Title

      Ann Thorac Med.

      Volume: 10 Pages: 61-65

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prognostic impact of cancer cachexia in patients with advanced non-small cell lung cancer.2015

    • Author(s)
      Kimura M, Naito T, Kenmotsu H, Taira T, Wakuda K, Oyakawa T, Hisamatsu Y, Tokito T, Imai H, Akamatsu H, Ono A, Kaira K, Murakami H, Endo M, Mori K, Takahashi T, Yamamoto N.
    • Journal Title

      Support Care Cancer

      Volume: 23

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of actionable mutations in malignant pleural mesothelioma2014

    • Author(s)
      Takehito Shukuya
    • Journal Title

      Lung Cancer

      Volume: 86 Issue: 1 Pages: 35-40

    • DOI

      10.1016/j.lungcan.2014.08.004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Multiplexed molecular profiling of lung cancer using pleural effusion2014

    • Author(s)
      Akamatsu H, Koh Y, Kenmotsu H, Naito T, Serizawa M, Kimura M, Mori K, Imai H, Ono A, Shukuya T, Taira T, Murakami H, Ohde Y, Endo M, Nakajima T, Takahashi T, Yamamoto N
    • Journal Title

      J Thorac Oncol

      Volume: 9 Pages: 1048-1052

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The effect of gefitinib in patients with postoperative recurrent non-small cell lung cancer harboring mutations of the epidermal growth factor receptor.2014

    • Author(s)
      Ko R, Kenmotsu H, Hisamatsu Y, Akamatsu H, Omori S, Nakashima K, Oyakawa T, Wakuda K, Shukuya T, Ono A, Imai H, Taira T, Naito T, Murakami H, Mori K, Endo M, Ohde Y, Takahashi K, Takahashi T.
    • Journal Title

      Int J Clin Oncol

      Volume: epub ahead of print

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of actionable mutations in malignant pleural mesothelioma.2014

    • Author(s)
      Shukuya T, Serizawa M, Watanabe M, Akamatsu H, Abe M, Imai H, Tokito T, Ono A, Taira T, Kenmotsu H, Naito T, Murakami H, Takahashi T, Endo M, Ohde Y, Nakajima T, Yamamoto N, Koh Y.
    • Journal Title

      Lung Cancer

      Volume: 86 Pages: 35-40

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Individual-level data on the relationships of progression-free survival and post-progression survival with overall survival in patients with advanced non-squamous non-small cell lung cancer patients who received second-line chemotherapy.2014

    • Author(s)
      Imai H, Mori K, Ono A, Akamatsu H, Taira T, Kenmotsu H, Naito T, Kaira K, Murakami H, Endo M, Nakajima T, Takahashi T.
    • Journal Title

      Med Oncol

      Volume: 31 Pages: 88-88

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Changes in forced expiratory volume in 1 second over time in patients with controlled asthma at baseline.2014

    • Author(s)
      Matsunaga K, Ichikawa T, Oka A, Morishita Y, Kanai K, Hiramatsu M, Akamatsu H, Kawabata H, Kikuchi T, Akamatsu K, Hirano T, Kou Y, Nakanishi M, Minakata Y, Yamamoto N.
    • Journal Title

      Respir Med

      Volume: 108 Pages: 976-982

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Toxicity and efficacy of chemotherapy for non-small cell lung cancer with cavitary lesions.2014

    • Author(s)
      Tokito T, Shukuya T, Akamatsu H, Ono A, Taira T, Kenmotsu H, Naito T, Murakami H, Takahashi T, Endo M, Mori K, Yamamoto N.
    • Journal Title

      Respir Investig

      Volume: 52

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Assessment of mutational profile of Japanese lung adenocarcinoma patients by multitarget assays: a prospective, single-institute study.2014

    • Author(s)
      Serizawa M, Koh Y, Kenmotsu H, Isaka M, Murakami H, Akamatsu H, Mori K, Abe M, Hayashi I, Taira T, Maniwa T, Takahashi T, Endo M, Nakajima T, Ohde Y, Yamamoto N.
    • Journal Title

      Cancer

      Volume: 15 Pages: 1471-81

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of cisplatin plus pemetrexed and cisplatin plus gemcitabine for the treatment of malignant pleural mesothelioma in Japanese patients.2014

    • Author(s)
      Shukuya T, Takahashi T, Imai H, Tokito T, Ono A, Akamatsu H, Taira T, Kenmotsu H, Naito T, Murakami H, Endo M, Yamamoto N.
    • Journal Title

      Respir Investig

      Volume: 52 Pages: 101-106

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Establishment of novel multiplexed assay to detect EGFR mutations using ultra-sensitive digital PCR2015

    • Author(s)
      赤松弘朗、洪泰浩、山本信之ら
    • Organizer
      ESMO Asia 2015
    • Place of Presentation
      Suntec, Singapore
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 胸部原発悪性腫瘍における個別化医療の現状と今後の展望について2015

    • Author(s)
      洪泰浩
    • Organizer
      第 74 回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Liquid biopsies: noninvasive longitudinal monitoring of lung cancer.2015

    • Author(s)
      洪泰浩
    • Organizer
      第 13 回日本臨床腫瘍学会学術集会
    • Place of Presentation
      ロイトン札幌
    • Year and Date
      2015-07-16
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Early Phase Trials Using Liquid Biopsy.2015

    • Author(s)
      赤松弘朗
    • Organizer
      4th Japan Taiwan Oncology Phase Ⅰ Trial Conference
    • Place of Presentation
      NTUH International Convention Center, Taipei, Taiwan
    • Year and Date
      2015-04-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Ongoing early phase trials using liquid biopsy2015

    • Author(s)
      Hiroaki Akamatsu
    • Organizer
      The 4th Japan-Taiwan Oncology Phase I Trial Conference
    • Place of Presentation
      International Conference Center of National Taiwan University Hospital
    • Year and Date
      2015-04-26
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Establishment of multiplexed ultra-sensitive detection of epidermal growth factor receptor mutations using picodroplet digital PCR2015

    • Author(s)
      Hiroaki Akamatsu, Yasuhiro Koh, Masaru Watanabe, Takashi Kikuchi, Masanori Nakanishi, Kazuto Matsunaga, Nobuyuki Yamamoto.
    • Organizer
      American Association of Cancer Research Annual Meeting 2015
    • Place of Presentation
      Pennsylvania Convention Center
    • Year and Date
      2015-04-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Establishment of multiplexed ultra-sensitive detection of epidermal growth factor receptor mutations using picodroplet digital PCR.2015

    • Author(s)
      赤松弘朗、洪泰浩、山本信之ら
    • Organizer
      American Association for Cancer Research Annual Meeting 2015
    • Place of Presentation
      Pennsylvania convention center, USA
    • Year and Date
      2015-04-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Droplet Digital PCR を用いた EGFR 遺伝子変異のマルチプレックス検出2014

    • Author(s)
      渡辺 勝、洪 泰浩、赤松 弘朗、菊池 崇史、松永 和人、山本 信之
    • Organizer
      日本肺癌学会総会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2014-11-15
    • Related Report
      2014 Research-status Report
  • [Presentation] Significance ofconsolidation chemotherapyin unresectable, locally advanced non-small cell lung cancer.2014

    • Author(s)
      Hiroaki Akamatsu
    • Organizer
      日本臨床腫瘍学会総会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2014-07-17
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] 呼吸器疾患診療最新ガイドライン2014

    • Author(s)
      菊池崇史, 赤松弘朗, 山本信之
    • Publisher
      総合医学社
    • Related Report
      2014 Research-status Report
  • [Book] がん薬物療法の作用機序 殺細胞性抗悪性腫瘍薬 白金製剤・アルキル化薬・抗がん性抗生物質2014

    • Author(s)
      赤松弘朗, 山本信之
    • Publisher
      日本臨床社
    • Related Report
      2014 Research-status Report
  • [Book] Ⅲ期非小細胞肺癌の化学放射線療法.2014

    • Author(s)
      赤松弘朗, 山本信之
    • Publisher
      へるす出版
    • Related Report
      2014 Research-status Report
  • [Book] EBMの手法による肺癌診療ガイドライン2014年版2014

    • Author(s)
      赤松弘朗, 山本信之
    • Publisher
      金原出版
    • Related Report
      2014 Research-status Report
  • [Book] 現在進行中の大規模無作為化比較試験2014

    • Author(s)
      赤松弘朗, 山本信之
    • Publisher
      南山堂
    • Related Report
      2014 Research-status Report
  • [Book] 腫瘍病理鑑別診断アトラス 肺癌2014

    • Author(s)
      赤松弘朗, 山本信之
    • Publisher
      文光堂
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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