| Project/Area Number |
26870546
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
Metabolomics
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 骨芽細胞系細胞 / 末梢血単核球 / 炎症性サイトカイン / 腫瘍壊死因子 / 関節リウマチ / 心血管疾患 / 動脈硬化 / 石灰化 / 骨芽細胞 / Wntシグナル / 動脈硬化症 |
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| Outline of Final Research Achievements |
Rheumatoid arthritis is a chronic systemic inflammatory disease with an increased risk of the developing cardiovascular diseases. A major risk factor for the cardiovascular diseases is vascular wall calcification, underlying molecular mechanisms of which remain as yet unclear. Recent studies have suggested that circulating osteoblast-lineage cells are associated with a marked induction of vascular wall calcification. Stimulation of peripheral blood mononuclear cells with TNFα or IL-6 formed mineralized nodules on dentin slices. These structures were calcified matrices composed of calcium and phosphate. Expression levels of osteoblastic markers in PBMCs by stimulation with TNFα or IL-6 was significantly up-regulated. Our results demonstrated that TNFα can induce the differentiation of human osteoblast-like cells from peripheral-blood mononuclear cells on dentin slices.
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