Analyses of candidate genes with multi-step DNA methylation alterations in lung cancer

• Project/Area Number: 26870569
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology; Respiratory organ internal medicine
• Research Institution: Keio University
• Principal Investigator: Sato Takashi 慶應義塾大学, 医学部, 共同研究員 (20464836)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肺がん / DNAメチル化 / エピジェネティクス

Outline of Final Research Achievements

Lung cancer is the leading cause of cancer-related death worldwide. It is known that epigenetic changes contribute to human carcinogenesis. We examined candidate genes which were obtained from genome-wide DNA methylation analysis. mRNA expression of PTPRH, one of the candidate genes, were upregulated in non-small cell lung cancer tissues compared with non-cancerous lung tissues and inversly correlated with DNA methylation levels in a cohort of lung adenocarcinoma patients. DNA-demethylating treatment of cell lines restored mRNA
PTPRH expression levels, suggesting that PTPRH gene expression was regulated by DNA hypomethylation. Furthermore, we identified the DNA methylation status of PTPRH as a novel prognostic factor for lung adenocarcinoma.

Research Products

• [Journal Article] Prognostic implication of PTPRH hypomethylation in non-small cell lung cancer (2015)
  • Author(s): Sato T, Soejima K, Arai E, Hamamoto J, Yasuda H, Arai D, Ishioka K, Ohgino K, Naoki K, Kohno T, Tsuta K, Watanabe S, Kanai Y and Betsuyaku T
  • Journal Title: Oncol Rep Volume: 34 Issue: 3 Pages: 1137-45
  • DOI: 10.3892/or.2015.4082
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Hypomethylation and prognostic implication of PTPRH in non-small cell lung cancer (2015)
  • Author(s): Sato T, Soejima K, Arai E, Hamamoto J, Yasuda H, Arai D, Ishioka K, Ohgino K, Naoki K, Kohno T, Tsuta K, Watanabe S, Kanai Y, Betsuyaku T
  • Organizer: IASLC 16th World Conference on Lung Cancer
  • Place of Presentation: Denver, Colorado, USA
  • Year and Date: 2015-09-06
  • Int'l Joint Research
• [Presentation] 非小細胞肺がんにおけるPTPRH のDNA 低メチル化と予後への関与 (2014)
  • Author(s): 佐藤崇、副島研造、新井恵吏、浜本純子、安田浩之、荒井大輔、石岡宏太、扇野圭子、猶木克彦、河野隆志、渡辺俊一、金井弥栄、別役智子
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2014-09-25 – 2014-09-27