| Project/Area Number |
26870594
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Chuo University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ナノチューブ / ドラックキャリアー / 遺伝子導入 / 交互積層 / DNA構造体 / ドラッグキャリア / 交互積層法 |
|---|
| Outline of Final Research Achievements |
Nanotubes (NTs) composed of DNA and polyethyleneimine were synthesized by alternating layer-by-layer assembly method using nanoporous polycarbonate membrane as a template. Doxorubicin (Dox), a DNA intercalating anticancer drug, can be loaded into the DNA layers of the NTs with high density. Dox-loaded DNA NTs were incorporated into the cancer cells and clearly showed anticancer activity. This activity was drug dose-dependent and slightly higher than that of Dox solution. Moreover, DNA NTs, prepared by using a plasmid DNA vector, successfully delivered the GFP (green fluorescent protein) gene into mammalian cells.
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