The association between antimicrobial resistance and the mutational ability of Streptococcus pneumoniae

• Project/Area Number: 26870612
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine; Infectious disease medicine
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: Wajima Takeaki 東京薬科大学, 薬学部, 助教 (00516669)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肺炎球菌 / 莢膜型 / ワクチン / 形質転換 / com

Outline of Final Research Achievements

This study aims to clarify the association between bacterial features and the transformation and mutational ability of Streptococcus pneumoniae strains. Strains with low transformation ability were detected among clinical isolates. These strains had an amino acid substitution in ComD, which is the receptor for the competence stimulating peptide. We developed a detection technique for this particular amino acid substitution using PCR that was subsequently used to screen clinical isolates. Results obtained in this study indicated that non-vaccine serotypes had a significantly lower transformation ability compared with that of vaccine serotypes.

Research Products

• [Journal Article] Host factors and biomarkers associated with poor outcomes in adults with invasive pneumococcal disease (2016)
  • Author(s): Hanada S., Iwata S., Kishi K., Morozumi M., Chiba N., Wajima T., Takata M., Ubukata K.
  • Journal Title: PLoS One Volume: 11 Issue: 1 Pages: e0147877-e0147877
  • DOI: 10.1371/journal.pone.0147877
  • Peer Reviewed / Open Access
• [Journal Article] Rapid Changes of Serotype and Genetic Drug Resistance in Isolates from Japanese Adults with Invasive Pneumococcal Diseases after PCV7 Introduction in Children, 2010-2013 (2015)
  • Author(s): Ubuakata K., Chiba N., Hanada S., Morozumi M., Wajima T., Shouji M., Iwata S., the IPD surveillance study group
  • Journal Title: Emerg. Infect. Dis. Volume: 21 Issue: 11 Pages: 1956-1965
  • DOI: 10.3201/eid2111.142029
  • Peer Reviewed / Open Access
• [Presentation] 肺炎球菌結合型ワクチン市販後における莢膜型の変化と新たに出現した無莢膜型株の特徴 (2016)
  • Author(s): 大里 隆二、輪島 丈明、野口 雅久
  • Organizer: 日本薬学会 第136年会
  • Place of Presentation: 横浜市
  • Year and Date: 2016-03-26
• [Presentation] 肺炎球菌ワクチン導入以降に分離された無莢膜型肺炎球菌の遺伝学的特徴 (2016)
  • Author(s): 輪島 丈明、野口 雅久
  • Organizer: 第89回 日本細菌学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2016-03-23
• [Presentation] 7価肺炎球菌結合型ワクチンの市販後における肺炎球菌の莢膜型の変化と薬剤感受性 (2015)
  • Author(s): 長谷部 太相, 輪島 丈明, 中南 秀将, 野口 雅久
  • Organizer: 日本薬学会 第135年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] Rapid changes in serotype and antimicrobial resistant profile of penicillin-nonsusceptible pneumococci by introduction of PCV7 (2014)
  • Author(s): T. Wajima, H. Nakaminami, K. Nakase, N. Noguchi
  • Organizer: International Conference on Antimicrobial Research 2014
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2014-10-01 – 2014-10-03