Identification of laminin active sites using functional peptides

• Project/Area Number: 26870613
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology; Biomolecular chemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: Katagiri Fumihiko 東京薬科大学, 薬学部, 助教 (60420642)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ペプチド / 細胞 / 受容体 / 線維芽細胞 / 遊走 / 接着

Outline of Final Research Achievements

Laminin is a huge protein around cells in the body, and it expresses a life phenomenon by giving various stimuli to the cell. To elucidate biological functions of laminin is thought to provide useful information for pharmaceutical field and regenerative medicine. The main biological functions of laminin are cell adhesion and cell motility. In this study, we evaluated cell adhesion and migration activity using synthetic peptides covering the amino acid sequence of laminin, and the receptor was identified.

Research Products

• [Journal Article] Biological activity of peptide-conjugated polyion complex matrices consisting of alginate and chitosan. (2017)
  • Author(s): Fujimori, C‡., Kumai, J‡., Nakamura, K., Gu, Y., Hozumi, K., Katagiri, F., Kikkawa, Y., and Nomizu, M
  • Journal Title: Biopolymers Volume: 108 Issue: 1 Pages: 356-366
  • DOI: 10.1002/bip.22983
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An anti-human lutheran glycoprotein phage antibody inhibits cell migration on laminin-511: epitope mapping of the antibody. (2017)
  • Author(s): Enomoto-Okawa Y, Maeda Y, Harashima N, Sugawara Y, Katagiri F, Hozumi K, Hui KM, Nomizu M, Ito Y, Kikkawa Y
  • Journal Title: PLoS One Volume: 12 Issue: 1 Pages: e0167860-e0167860
  • DOI: 10.1371/journal.pone.0167860
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effect of spacer length and type on the biological activity of peptide-polysaccharide matrices. (2016)
  • Author(s): Kumai, J, Hozumi, K, Yamada, Y, Katagiri, F, Kikkawa, Y, Nomizu, M
  • Journal Title: Biopolymers Volume: 印刷中 Issue: 4 Pages: 512-520
  • DOI: 10.1002/bip.22785
  • Peer Reviewed / Open Access
• [Journal Article] Down-regulation of cell adhesion via rho-associated protein kinase (ROCK) pathway promotes tumor cell migration on laminin-511 (2016)
  • Author(s): Kikkawa Y, Harashima N, Ikari K, Fujii S, Katagiri F, Hozumi K, Nomizu M
  • Journal Title: Exp Cell Res Volume: 344 Issue: 1 Pages: 76-85
  • DOI: 10.1016/j.yexcr.2016.04.002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mixed Fibronectin-Derived Peptides Conjugated to a Chitosan Matrix Effectively Promotes Biological Activities through Integrins, α4β1, α5β1, αvβ3, and Syndecan. (2016)
  • Author(s): Kentaro Hozumi, Kyotaro Nakamura, Haruna Hori, Mari Miyagi, Rika Nagao, Keiko Takasaki, Fumihiko Katagiri, Yamato Kikkawa, and Motoyoshi Nomizu
  • Journal Title: Biores Open Access Volume: 5 Issue: 1 Pages: 356-366
  • DOI: 10.1089/biores.2016.0037
  • Peer Reviewed / Open Access
• [Journal Article] Structural study of cell attachment peptide derived from laminin by molecular dynamics simulation. (2016)
  • Author(s): Yamada, H, Mori, S, Miyakawa, T, Morikawa, R, Katagiri, F, Hozumi, K, Kikkawa, Y, Nomizu, M, Takasu, M
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0149474-e0149474
  • DOI: 10.1371/journal.pone.0149474
  • Peer Reviewed / Open Access
• [Journal Article] Suppression of cell adhesion through specific integrin crosstalk on mixed peptide-polysaccharide matrices (2015)
  • Author(s): Kentaro Hozumi, Chikara Fujimori, Fumihiko Katagiri, Yamato Kikkawa, Motoyoshi Nomizu
  • Journal Title: Biomaterials Volume: 37 Pages: 73-81
  • DOI: 10.1016/j.biomaterials.2014.10.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Biological activities of the homologous loop regions in the laminin alpha chain LG modules. Biochemistry. (2014)
  • Author(s): Katagiri, F., Hara, T., Yamada, Y., Urushibata, S., Hozumi, K., Kikkawa, Y., Nomizu, M.
  • Journal Title: Biochemistry Volume: 53 Issue: 22 Pages: 3699-3708
  • DOI: 10.1021/bi5003822
  • Peer Reviewed
• [Journal Article] Screening of integrin-binding peptides in a laminin peptide library derived from the mouse laminin β chain short arm regions (2014)
  • Author(s): Katagiri F, Takagi M, Nakamura M, Tanaka Y, Hozumi K, Kikkawa Y, Nomizu M
  • Journal Title: Arch Biochem Biophys Volume: 550-551 Pages: 33-41
  • DOI: 10.1016/j.abb.2014.04.008
  • Peer Reviewed
• [Journal Article] Soluble lutheran/basal cell adhesion molecule is detectable in plasma of hepatocellular carcinoma patients and modulates cellular interaction with laminin-511 in vitro (2014)
  • Author(s): Kikkawa Y, Miwa T, Tanimizu N, Kadoya Y, Ogawa T, Katagiri F, Hozumi K, Nomizu M, Mizuguchi T, Hirata K, Mitaka T
  • Journal Title: Experimental Cell Research Volume: 328 Issue: 1 Pages: 197-206
  • DOI: 10.1016/j.yexcr.2014.07.012
  • Peer Reviewed / Open Access
• [Presentation] アミロイド様線維形成ラセミ混合ペプチドの生物活性 (2017)
  • Author(s): 片桐 文彦
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-24
• [Presentation] The phosphorylation of focal adhesion kinase in human dermal fibroblasts on chiral/racemic amyloid-like fibrils (2016)
  • Author(s): F. Katagiri
  • Organizer: 2016 Annual Meeting the American Society for Cell Biology
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Amyloid-like fibril formation and biological activity of racemic fibrogenic peptides (2016)
  • Author(s): F. Katagiri
  • Organizer: 第53回ペプチド討論会
  • Place of Presentation: 京都
  • Year and Date: 2016-10-26
• [Presentation] EFFECT OF RACEMIC AMYLOIDOGENIC PEPTIDES ON PHOSPHOLYLATION OF FOCAL ADHESION KINASE Effect of racemic amyloidogenic peptides on phospholylation of focal adhesion kinase (2016)
  • Author(s): F. Katagiri
  • Organizer: 34th Europian Petide symposium
  • Place of Presentation: Leipzig (Germany)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] ラミニンγ1鎖配列由来生物活性ペプチドC16の構造活性相関研究 (2016)
  • Author(s): 片桐 文彦
  • Organizer: 日本薬学会136年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-26
• [Presentation] Structure-activity relationship study for biologically active peptide C16 (KAFDITYVRLKF) derived from mouse laminin γ1 chain sequence (2015)
  • Author(s): Fumihiko Katagiri
  • Organizer: ASCB2015
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-12-12
  • Int'l Joint Research
• [Presentation] Structural Requirement of Fibrogenic Peptide AG97 (SAKVDAIGLEIV) and B160 (VILQQSAADIAR) for Amyloid-Like Fibril Formation and Cellular Activity (2015)
  • Author(s): Fumihiko Katagiri
  • Organizer: American Peptide Symposium 2015
  • Place of Presentation: Orlando,USA
  • Year and Date: 2015-06-20
  • Int'l Joint Research
• [Presentation] インテグリンα6β1結合ペプチドA2G10の構造活性相関研究 (2015)
  • Author(s): 片桐 文彦、山中 涼、陶山 竜作、中島 直美、山田 雄二、保住 建太郎、吉川 大和、野水 基義
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] Structure-activity relationship study for integrin alpha6beta1-binding peptide A2G10 derived from mouse laminin alpha2 chain sequence. (2014)
  • Author(s): F. Katagiri, R. Yamanaka, R. Suyama, N. Nakajima, Y. Yamada, K. Hozumi, Y. Kikkawa, M. Nomizu.
  • Organizer: The 2014 ASCB/IFCB meeting
  • Place of Presentation: フィラデルフィア、アメリカ合衆国
  • Year and Date: 2014-12-06 – 2014-12-10
• [Presentation] Identification of integrin-binding sites on short arm region of laminin alpha5 chain by screening in synthetic peptide library. (2014)
  • Author(s): F. Katagiri, H. Shirono, K. Kobayashi, K. Hozumi, Y. Kikkawa, M. Nomizu.
  • Organizer: 33rd European Peptide Symposium
  • Place of Presentation: ソフィア、ブルガリア
  • Year and Date: 2014-08-31 – 2014-09-05
• [Remarks] 東京薬科大学薬学部病態生化学教室
  • URL: http://www.ps.toyaku.ac.jp/~nomizu/index.html
• [Remarks] researchmap
  • URL: http://researchmap.jp/fumihikokatagiri/
• [Remarks] 病態生化学教室
  • URL: http://www.ps.toyaku.ac.jp/~nomizu/index.html
• [Remarks] researchmap
  • URL: http://researchmap.jp/FumihikoKatagiri/