Vascular patterning related with the hindbrain in zebrafish embryo

• Project/Area Number: 26870618
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology); Developmental biology
• Research Institution: Kanagawa University (2016); Toyo University (2014-2015)
• Principal Investigator: Fujita Misato 神奈川大学, 理学部, 助教 (60633550)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 脳血管 / ガイダンス因子 / 後脳 / 発生 / ゼブラフィッシュ / 血管 / 器官形成 / 脳 / 期間形成

Outline of Final Research Achievements

Previous studies have revealed that the developmental process of the vascular system follows a reproducible stereotypic program in each species. From the comparative anatomical point of view, a common developmental pattern is highly conserved throughout the vertebrates during early development. The brain vessels have an essential role for survival commonly in all vertebrates and in this study zebrafish was used for the research of brain vascular patterning.
The rhombomere is a common segmental structure in the developing hindbrain. Each center of rhombomere is characterized as a signaling center and determine neuronal development. The molecular mechanisms for the relationship between the vascular patterning and neuronal/non-neuronal development has not been uncovered. I examined that guidance cue(s) from each area in rhombomere and carried out gain- and loss- of function to figure out the relationship between hindbrain central arteries and rhombomere area-specific cell clusters.

Research Products

• [Presentation] Assembly and patterning of the vascular network of the zebrafish hindbrain (2017)
  • Author(s): Misato Fujita
  • Organizer: 神奈川大学・国立台湾大学国際交流学術会議
  • Place of Presentation: 国立台湾大学
  • Year and Date: 2017-03-10
  • Invited
• [Presentation] Patterning of the central arteries in the zebrafish hindbrain (2016)
  • Author(s): Misato Fujita
  • Organizer: 第24回日本血管生物医学会学術集会
  • Place of Presentation: 長崎ブリックホール
  • Year and Date: 2016-12-08
• [Presentation] Differentiation of the Vascular Endothelial Cells in the Developing Zebrafish (2015)
  • Author(s): Misato Fujita, Erina Saito, Sumio Isogai
  • Organizer: 9th European Zebrafish Meeting
  • Place of Presentation: Oslo Congress Centre（ノルウェー）
  • Year and Date: 2015-06-28
  • Int'l Joint Research