| Project/Area Number |
26870630
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
Integrative animal science
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| Research Institution | Meiji University |
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Principal Investigator |
Matsunari Hitomi 明治大学, 研究・知財戦略機構, 特任講師 (70639517)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 異種移植 / ブタ / ゲノム編集 / 体細胞核移植 / 臓器移植 / 遺伝子ノックアウト / 移植・再生医療 / トランスレーショナルリサーチ |
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| Outline of Final Research Achievements |
Pig to human xenotransplantation is one of the current strategies for overcoming the worldwide shortage of organ transplants. However, discordant xenografts involve severe immune rejections strongly related to species-specific glycoantigens. The major xenoantigen is the α-Gal epitope. Another carbohydrate xenoantigen, N-glycolylneuraminic acid (Neu5Gc), which is a component of the H-D antigen, has also been identified. Neu5Gc is synthesized by cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH). The aim of this study was to produce α1,3-galactosyltransferase (GalT)/CMAH-double KO pigs using genome editing and somatic cell nuclear transfer (SCNT) technology. The homozygous CMAH KO cells were established using ZFNs or TALENs. These cells were used for SCNT and produced GalT/CMAH-double KO cloned pigs. The absence of α-Gal and H-D antigens was confirmed in both the tissue and granulocyte samples of the cloned piglets.
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