Identification of genes that play an important role in tooth root morphogenesis

• Project/Area Number: 26870666
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry; Orthodontics/Pediatric dentistry
• Research Institution: Tsurumi University
• Principal Investigator: TASHIMA Itaru 鶴見大学, 歯学部, 学部助手 (10612004)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 遺伝子 / マウス / 樋状根 / in situ hybridization / 遺伝子発現 / 歯根の形態形成 / SHH signaling pathway

Outline of Final Research Achievements

The aim of this study was to identify the genes for influencing to tooth root form using C57L/J strain mice(LJ mice) and C57BL/6J strain mice(B6 mice). We performed in situ hybridization to analyze the expression patterns of gene belong to sonic hedgehog(SHH) signaling pathway in postnatal days 10, 11, 12 of mice.
Expression patterns of SHH, Ptc1 and Gli1 showed differences in tooth root bifurcation area between LJ mice and B6 mice. It is suggested that SHH signaling pathway is involved to tooth root formation in C57L/J strain mice.