| Project/Area Number |
26870677
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pharmacology in pharmacy
General pharmacology
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 細胞内亜鉛 / TRP channel / 亜鉛トランスポータ / TRPチャネル |
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| Outline of Final Research Achievements |
We established a system to measure the extracellular zinc influx through zinc transporters in the HEK293 cells overexpressing Zip8 (HEK-Zip8) and Zip14 (HEK-Zip14) using a zinc-sensitive dye FluoZin-3. The extracellular zinc influx in the HEK-Zip8 and HEK-Zip14 were quantitatively measured by quantifying the extracellular zinc content in the medium.
The effects of polaprezinc, a chelate compound of zinc ion, were examined using both HEK293 cells overexpressing TRPA1 (HEK-TRPA1) and human fibroblast-like synoviocytes stimulated by IL-1alpha. The polaprezinc increased intracellular Ca2+ concentration in both HEK-TRPA1 and IL-1alpha-stimulated synoviocytes. Moreover, the effects of a captopril that cause taste disorder were examined. The captopril did not change the response of polaprezinc in the IL-1alpha-stimulated synoviocytes. These results suggest that polaprezinc is effective as a zinc supplementation drug, and the zinc-chelating effect of captopril is very weak.
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