| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
The anti-oxidant responsive element (ARE)-mediated transcription is a key event in cellular detoxification of electrophilic exnobiotics. We previously established a clonal cell line stably transfected with an ARE-destabilized luciferase reporter gene, and showed that 2-hydroxyethyl methacrylate (HEMA), which is more toxic than methyl methacrylate (MMA), highly increased ARE-mediated trancriptional activity at low concentrations than MMA. We here showed the dose-dependent effects of ethyl methacrylate (EMA) on ARE activity and their relation to cytotoxicity. These results showed that the low concentration effects of resin monomers on ARE activity reflect their cytotoxicity probably due to their electroreactivity to intracellular molecules.
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