| Project/Area Number |
26870692
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
Experimental pathology
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| Research Institution | Niigata University (2015)
Aichi Medical University (2014) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 細胞極性 / 腫瘍 / 浸潤 / 転移 / ガングリオシド / シアリダーゼ / ノイラミニダーゼ / 腫瘍形成 |
|---|
| Outline of Final Research Achievements |
The function of Crumbs3 (Crb3)in human tumorigenesis is not fully understood. Western blots and immunohistochemistry revealed that Crb3 is strongly expressed in a part of human adenocarcinoma cell lines, and is localized in the luminal plasma membrane not only in normal tissue but also in tubular cancers. Crb3 knockout (KO) colon cancer cell lines are established by CRISPR/CAS9 system, and Crb3 KO cells showed remarkable reduction in cell migration in vitro and in vivo. Ganglioside has been shown previously to suppress tumorigenesis by inhibiting plasma membrane receptors, and we found that ganglioside was increased on plasma membrane in Crb3 KO cells. Restoration of Crb3 in KO cell blocked plasma membrane localization of ganglioside. These data indicate that Crb3 suppresses ganglioside localization to plasma membrane, to inhibit tumor cell behaviors.
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