Novel insights into the expression and function of heat shock protein Hsp105 in tumor tissues and cells

• Project/Area Number: 26870701
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics; Biological pharmacy
• Research Institution: Kyoto Pharmaceutical University
• Principal Investigator: SAITO YOUHEI 京都薬科大学, 薬学部, 助教 (90411032)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 熱ショックタンパク質 / 腫瘍マーカー / 核内発現 / Hsp105 / Hsp70 / Stat3 / HIF-1

Outline of Final Research Achievements

We focused and analyzed the expression and localization of Hsp105 in tumor tissues. Immunohistochemical analysis revealed the overexpression of Hsp105 in nucleus of some tumor tissues. The findings using cell lines indicated the possibility that Hsp105 localizes in the nucleus and affects the expression and activation of anti-cancer target proteins. Although further studies are needed, these observations will provide novel strategy for the Hsp105-targeted anti-cancer therapy and diagnosis.

Research Products

• [Journal Article] Requirement of Hsp105 in CoCl2-induced HIF-1α accumulation and transcriptional activation (2017)
  • Author(s): Mikami H, Saito Y, Okamoto N, Kakihana A, Kuga T, Nakayama Y
  • Journal Title: Experimental Cell Research Volume: 352 Issue: 2 Pages: 225-233
  • DOI: 10.1016/j.yexcr.2017.02.004
  • Peer Reviewed
• [Journal Article] Yeast two-hybrid and one-hybrid screenings identify regulators of hsp70 gene expression (2016)
  • Author(s): Saito Y, Nakagawa T, Kakihana A, Nakamura Y, Nabika T, Kasai M, Takamori M, Yamagishi N, Kuga T, Hatayama T, Nakayama Y.
  • Journal Title: Journal of Cellular Biochemistry. Volume: in press Issue: 9 Pages: 2109-2117
  • DOI: 10.1002/jcb.25517
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nmi interacts with Hsp105beta and enhances the Hsp105beta-mediated Hsp70 expression (2014)
  • Author(s): Saito Y, Yukawa A, Matozaki M, Mikami H, Yamagami T, Kuga T, Yamagishi N, Hatayama T, Nakayama Y
  • Journal Title: Experimental Cell Research Volume: 327 Issue: 1 Pages: 163-170
  • DOI: 10.1016/j.yexcr.2014.07.023
  • Peer Reviewed
• [Presentation] HIF-1の蓄積および転写活性化にはHsp105が必要である (2017)
  • Author(s): 三上大貴、齊藤洋平、岡本菜美子、久家貴寿、中山祐治
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-24
• [Presentation] 熱ショックタンパク質Hsp105αの核局在化と抗がん剤抵抗性への寄与 (2016)
  • Author(s): 齊藤洋平、山根鉄平、島田雅史、加藤圭穂、久家貴寿、中山祐治
  • Organizer: 第39回 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-30
• [Presentation] Hsp70誘導および温熱感受性におけるサイトカインシグナル伝達系転写因子Stat3の関与 (2016)
  • Author(s): 齊藤洋平, 的崎雅史, 湯川明久, 多田円香, 久家貴寿, 中山祐治
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-26
• [Presentation] 抗がん剤抵抗性に及ぼすHsp105αの核局在化の関与 (2016)
  • Author(s): 齊藤洋平、山根鉄平、島田雅史、加藤圭穂、久家貴寿、中山祐治
  • Organizer: 第63回 日本生化学会 近畿支部例会
  • Place of Presentation: 神戸
• [Presentation] 低酸素誘導因子HIF-1の発現および転写活性化における熱ショックタンパク質Hsp105の関与 (2016)
  • Author(s): 三上大貴、齊藤洋平、久家貴寿、中山祐治
  • Organizer: 第66回 日本薬学会近畿支部総会・大会
  • Place of Presentation: 大阪
• [Presentation] 熱ショックによるサイトカインシグナル伝達系転写因子Stat3の活性化 (2015)
  • Author(s): 齊藤洋平, 的崎雅史, 湯川明久, 多田円香, 久家貴寿, 山岸伸行, 中山祐治
  • Organizer: BMB2015 第38回日本分子生物学会年会／第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] 齊藤洋平、湯川明久、的崎雅史、三上大貴、山岸伸行、久家貴寿、中山祐治 (2015)
  • Author(s): NmiによるStat3活性化を介したHsp70発現誘導
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 熊本
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 熱ショックタンパク質Hsp105のアドリアマイシンによる核局在化メカニズム (2014)
  • Author(s): 山根鉄平, 齊藤洋平, 島田雅史, 加藤圭穂, 久家貴寿, 山岸伸行, 中山祐治
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Nmi enhances the Hsp105β-mediated Hsp70 expression through the Stat signaling pathway (2014)
  • Author(s): Youhei Saito, Akihisa Yukawa, Masashi Matozaki, Hiroki Mikami, Nobuyuki Yamagishi, Takahisa Kuga, Yuji Nakayama
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27