Improvement of therapeutic resistance of glioma stem cells by 5-ALA photodynamic therapy

• Project/Area Number: 26870722
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics; Neurosurgery
• Research Institution: Osaka Medical College
• Principal Investigator: Masubuchi Takahiro 大阪医科大学, 医学部, 非常勤医師 (90512103)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 光線力学治療 / 光線力学診断 / 5－アミノレブリン酸 / ポルフィリン / ABCG2 / 腫瘍幹細胞 / 悪性神経膠腫 / 脳腫瘍 / 光線力学療法 / アミノレブリン酸 / プロトポルフィリンIX / ５アミノレブリン酸 / 5アミノレブリン酸 / がん幹細胞 / 治療抵抗性

Outline of Final Research Achievements

Terapeutic resistance to radiation therapy and chemotherapy of glioma stem cells is a major hurdle in the treatment of malignant glioma. In this study, ALA-PDT was performed using glioma stem cell lines (3 cell lines of mesenchymal type and 2 cell lines of proneural type). As a result, it was found that ALA-PDT is more sensitive to glioma stem cells and has superior characteristics such as decreased stemness after PDT treatment.