| Project/Area Number |
26870731
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
Applied microbiology
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| Research Institution | Kindai University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | イオン液体 / Bacillus 属細菌 / イオン液体耐性細菌 / 浸透圧ストレス / ゲノム解析 / 次世代シーケンサー / イオン液体耐性酵素 / プロテアーゼ / イオン液体耐性 / [BMIM][Cl] / 耐性酵素 / Bacillus属細菌 |
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| Outline of Final Research Achievements |
Ionic liquids are various kinds of molten organic salts, which are composed of a bulky asymmetric cation and a small anion. Extensive studies of biocatalytic reactions using ionic liquids have been carried out. An ionic liquid [BMIM][Cl] improved the digestibility of biomasses but was toxic to the bacteria. [BMIM][Cl] deactivated various enzymes. Therefore, there exists demand for a [BMIM][Cl]-tolerant bacterial host and an enzyme. In this study, Bacillus amyloliquefaciens CMW1 was isolated from a Japanese fermented soybean paste. Strain CMW1 grows in the presence of 10% (v/v, 631 mM) [BMIM][Cl], which inhibits the growth of various microorganisms. The genome sequence of strain CMW1 was determined and allowed for characterization of the molecular mechanism of the ionic liquid tolerance. A protease from strain CMW1 functioned in various hydrophilic and hydrophobic ionic liquids, and exhibited tolerance to ionic liquid and halo, alkaline, and organic solvents.
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