Development of novel therapy for intractable dermatitis using immunomodulator FTY720 (fingolimod) ointment

• Project/Area Number: 26870739
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics; Dermatology
• Research Institution: Setsunan University
• Principal Investigator: Tsui Takumi 摂南大学, 薬学部, 講師 (90454652)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: FTY720 / アトピー性皮膚炎 / 皮膚バリア機能

Outline of Final Research Achievements

We investigated the therapeutic efficacy of immunomodulator FTY720 ointment (fingolimod) for mite-induced intractable AD using an NC/Nga mouse model. Female NC/Nga mice that developed severe AD were divided into four groups: (1) FTY720, (2) tacrolimus, (3) betamethasone and (4) ointment base, all of which received treatment six times per week. Therapeutic efficacy after two weeks was evaluated in terms of AD severity, histochemical observations, transepidermal water loss (TEWL) and epidermal barrier function.
In the FTY720 group, AD improved significantly compared with the ointment base group, as did epidermal hypertrophy, mast cell accumulation, and CD3+ T cell infiltration. Furthermore, in the FTY720 group, TEWL decreased significantly compared with the ointment base group, and filaggrin expression significantly increased compared with the betamethasone and ointment base groups. FTY720 ointment is a promising candidate for treatment of intractable AD.

Research Products

• [Journal Article] Therapeutic approach to mite-induced intractable dermatitis using novel immunomodulator FTY720 ointment (fingolimod) in NC/Nga mice (2016)
  • Author(s): Takumi Tsuji, Satoshi Okuno, Ayano Kuroda, Junya Hamazaki, Takuma Chikami, Sakura Sakurai, Yuya Yoshida, Rie Banno, Tetsuro Fujita, Takeyuki Kohno
  • Journal Title: Allergology International Volume: 65 Issue: 2 Pages: 172-179
  • DOI: 10.1016/j.alit.2015.10.009
  • NAID: 130005149283
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] フィンゴリモド(FTY720)軟膏がアトピー性皮膚炎モデルマウスの皮膚バリア機能に与える影響 -第2報- (2016)
  • Author(s): 櫻井さくら, 千頭拓馬, 黒田綾乃，濱崎淳哉，辻琢己, 吉田侑矢, 坂野理絵, 藤多哲朗, 河野武幸
  • Organizer: 日本薬学会 第136年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-27
• [Presentation] フィンゴリモド（FTY720）軟膏がアトピー性皮膚炎モデルマウスの皮膚バリア機能に与える影響 (2014)
  • Author(s): 濱崎淳哉，奥野聡之，黒田綾乃，櫻井さくら，千頭拓馬，辻琢己，吉田侑矢，坂野理絵，藤多哲朗，河野武幸
  • Organizer: 第64回日本薬学会近畿支部大会
  • Place of Presentation: 京都薬科大学（京都）
  • Year and Date: 2014-10-11
• [Remarks] 摂南大学薬学部病態医科学研究室ホームページ
  • URL: http://www.setsunan.ac.jp/~p-rinsho/
• [Remarks] 摂南大学病態医科学研究室
  • URL: http://www.setsunan.ac.jp/~p-rinsho/gakkai2.html