Effect and mechanism of progesterone and attachment in endometriosis.

Research Project

Project/Area Number	26870783
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Endocrinology Obstetrics and gynecology
Research Institution	Fukuoka University
Principal Investigator	ITOH HIROKO 福岡大学, 医学部, 講師 (30632161)
Project Period (FY)	2014-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords	子宮内膜症 / プロゲステロン / TGFβ1 / MMP / ＴＧＦβ1 / ＭＭＰ
Outline of Final Research Achievements	Invasion of ectopic endometrium into peritoneal mesothelium requires matrix metalloproteinases (MMPs) for tissue remodeling. Here, we tested the hypothesis that TGFβ1 modifies progesterone-induced effects on the secretion of MMPs in normal and ectopic endometrium.The results indicate that MMP2 and MMP9 are not increased by TGFβ1 in endometrioma stromal cells. Although progesterone prevents attachment of endometrioma cells to matrix components of the abdominal wall, it does not ameliorate these abnormal stromal cell responses to TGFβ1. These data support that TGFβ1 has endogenous antiprogestational effects in HESC and ectopic endometrium from endometriosis. We suggest that the opposing effects of progesterone and TGFβ1 are important in regulation of matrix integrity in HESC but that ectopic endometrium is refractory to regulation of MMP activity by progesterone.