| Project/Area Number |
26870796
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
Developmental biology
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
Reina Komiya 沖縄科学技術大学院大学, サイエンステクノロジーグループ, サイエンス・テクノロジー アソシエート (90631416)
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| Research Collaborator |
Le Tu Ngoc
Ohouanagi Hajime
Nonomura Ken-ichi
Endo Masaki
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 長鎖non-coding RNA / small RNA / 非コードゲノム / イネ / 生殖 / non-coding RNA / ノンコーディングRNA |
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| Outline of Final Research Achievements |
Successful sexual reproduction in flowering plants depends on accurate germ cell differentiation. Molecular mechanisms of germ cell development during pre-meiotic stages remain unknown in plants. I have identified over 700 types of long intergenic non-coding RNAs (lincRNAs) specifically expressed during rice reproductive stages. These lincRNAs that contain the consensus sequences of microRNA 2118 (miR2118), are precursors of 21-nucleotides small RNAs (Komiya et al., 2014, Komiya 2017). Furthermore, I am engaged in two main projects using rice: 1. Roles of over 700 lincRNAs and 21-nt small RNAs in early reproduction and 2. Transcriptional regulation of 700 lincRNAs by CRF1. Some lincRNA mutants and crf1 mutants showed sterility. Furthermore, some lincRNAs expressions are suppressed in crf mutants. These results suggest that multiple lincRNAs may regulate early reproduction and CRF is required for development of rice reproductive tissues (Komiya unpublished data).
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