| Project/Area Number |
26870824
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ecology/Environment
Evolutionary biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
Ishikawa Asano 国立遺伝学研究所, 集団遺伝研究系, 助教 (20722101)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 日長応答性 / 甲状腺刺激ホルモン / 季節繁殖 |
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| Outline of Final Research Achievements |
Although loss of seasonality has been found throughout the animal kingdom, we know little about its molecular and genetic mechanisms. We are addressing this question using marine and stream ecotypes of sticklebacks. To investigate the mechanisms underlying the divergence in photoperiodic responses between the ecotypes, we conducted microarray analysis and found that photoperiodic response of TSHb2 gene was lost in stream. Reduction of TSHb2 response was found in two independently derived stream populations from Japan and Canada. TSHb2-knockdown fish showed that TSHb2 suppresses LH and FSH expressions and testis development under short days. In the Canada, cis mutations contributed to the loss of TSHb2 response, whereas in Japan it was highly polygenic. Genome sequencing revealed several mutations at the cis region of the Canadian stream TSHb2 locus. RNAseq of pituitary revealed several transcription factors that show expression changes immediately after the photoperiodic shift.
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