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Regulatory mechanism of delipidation of Atg8 that mediates autophagosome formation

Research Project

Project/Area Number 26870828
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Physical pharmacy
Research InstitutionMicrobial Chemistry Research Foundation

Principal Investigator

FUJIOKA Yuko (NODA Yuko)  公益財団法人微生物化学研究会, 微生物化学研究所, 研究員 (80399964)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsオートファジー / X線結晶構造解析
Outline of Final Research Achievements

Atg8, a ubiquitin-like protein important for membrane dynamics during autophagy, is modified with a phospholipid and localizes to the membrane, thereby contributes to the progression of autophagy. Atg4 is a deconjugating enzyme for lapidated Atg8 and contributes to the recycling of Atg8; however, the regulation mechanism of Atg4 activity remained elusive. Here, I studied the components that regulate Atg4 activity in vitro. The data showed that the activity of Atg4 was affected by neither the interaction with other Atg proteins nor Atg1-mediated phosphorylation, but by the regulatory regions within Atg4. These data suggested that Atg4 might possess an ability to regulate its deconjugating activity depending on the intracellular localization.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015 2014 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] Structural biology of the core autophagy machinery.2017

    • Author(s)
      Suzuki H, Osawa T, Fujioka Y, Noda NN
    • Journal Title

      Current Opinion in Structural Biology

      Volume: 43 Pages: 10-17

    • DOI

      10.1016/j.sbi.2016.09.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The Intrinsically Disordered Protein Atg13 Mediates Supramolecular Assembly of Autophagy Initiation Complexes.2016

    • Author(s)
      Yamamoto H, Fujioka Y, Suzuki SW, Noshiro D, Suzuki H, Kondo-Kakuta C, Kimura Y, Hirano H, Ando T, Noda NN, Ohsumi Y
    • Journal Title

      Developmental Cell

      Volume: 38 Issue: 1 Pages: 86-99

    • DOI

      10.1016/j.devcel.2016.06.015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural basis of the differential function of the two C. elegans Atg8 homologs, LGG-1 and LGG-2, in autophagy.2015

    • Author(s)
      Wu, F., Watanabe, Y., Guo, X. Y., Qi, X., Wang, P., Zhao, H. Y., Wang, Z., Fujioka, Y., Zhang, H., Ren, J. Q., Fang, T. C., Shen, Y. X., Feng, W., Hu, J. J., Noda, N. N., Zhang, H.
    • Journal Title

      Molecular Cell

      Volume: 60 Issue: 6 Pages: 914-929

    • DOI

      10.1016/j.molcel.2015.11.019

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Atg1 family kinases in autophagy initiation.2015

    • Author(s)
      Noda, N. N., Fujioka, Y.
    • Journal Title

      Cellular and Molecular Life Sciences

      Volume: 72 Issue: 16 Pages: 3083-3096

    • DOI

      10.1007/s00018-015-1917-z

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural Basis of Starvation-induced Assembly of the Autophagy Initiation Complex2015

    • Author(s)
      藤岡優子、野田展生
    • Journal Title

      Nihon Kessho Gakkaishi

      Volume: 57 Issue: 3 Pages: 191-197

    • DOI

      10.5940/jcrsj.57.191

    • NAID

      130005085906

    • ISSN
      0369-4585, 1884-5576
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural basis of starvation-induced assembly of the autophagy initiation complex2014

    • Author(s)
      Yuko Fujioka, Sho W Suzuki, Hayashi Yamamoto, Chika Kondo-Kakuta, Yayoi Kimura, Hisashi Hirano, Rinji Akada, Fuyuhiko Inagaki, Yoshinori Ohsumi, Nobuo N Noda
    • Journal Title

      NATURE STRUCTURAL & MOLECUIAR BIOLOGY

      Volume: 21(発表確定) Issue: 6 Pages: 513-521

    • DOI

      10.1038/nsmb.2822

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Structural basis of the pre-autophagosomal structure assembly mediated by an intrinsically disordered protein Atg132016

    • Author(s)
      藤岡優子,山本林,鈴木翔,大隅良典,野田展生
    • Organizer
      内藤コンファレンス「生命科学に革命をもたらす最先端構造生物学」
    • Place of Presentation
      シャトレーゼガトーキングダムサッポロ(北海道 札幌市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] プレオートファゴソーム構造体の中核複合体のin vitro再構成と性状解析2015

    • Author(s)
      藤岡優子, 山本 林, 鈴木 翔, 大隅良典, 野田展生
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド(兵庫県 神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] PASの中核複合体のin vitro再構成と溶液構造解析2015

    • Author(s)
      藤岡優子, 山本 林, 鈴木 翔, 能代大輔,有坂文雄, 安藤敏夫,大隅良典, 野田展生
    • Organizer
      第9回オートファジー研究会
    • Place of Presentation
      淡路夢舞台(兵庫県 淡路市)
    • Year and Date
      2015-11-16
    • Related Report
      2015 Research-status Report
  • [Presentation] Molecular mechanism of PAS (pre-autophagosomal structure) organization.2014

    • Author(s)
      Fujioka Y, Suzuki SW, Yamamoto H, Kondo-Kakuta C, Kimura Y, Hirano H, Akada R, Inagaki F, Ohsumi Y, Noda NN.
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      ワークピア横浜/横浜産貿ホールマリネリア(横浜)
    • Year and Date
      2014-06-25
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 微生物化学研究所HP

    • URL

      http://www.bikaken.or.jp/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 微生物化学研究所 HP

    • URL

      http://www.bikaken.or.jp/

    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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