| Project/Area Number |
26870842
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
Animal production science
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
Yamamura Takashi 国立研究開発法人農業生物資源研究所, 動物生産生理機能研究ユニット, 主任研究員 (60582723)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | GnRH / パルス状分泌 / ニューロキニン / キスペプチン / ターゲットトキシン |
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| Outline of Final Research Achievements |
Kisspeptin/neurokinin B (NKB)/dynorphin (KNDy) neurons of the arcuate nucleus (ARC) play a key role in control of pulsatile GnRH secretion. Recent evidence suggests that NKB plays a pivotal role in GnRH pulse generation. Firstly we found that NK3R, which is one of neurokinin receptors and preferentially binds to NKB, plays a predominant role in GnRH pulse generation of goats. Second, to study NK3R function in GnRH pulse generation, we used a method to selectively ablate KNDy neurons using NK3-SAP, a NK3R agonist conjugated to saporin. NK3-SAP was microinjected into the ARC of goats using stereotaxic surgery. After 3 weeks of injections, we took a blood sample for measurement of LH levels and perfused for immunocytochemistry for kisspeptin in the ARC. NK3-SAP treatments tended to reduce amplitude of LH pulses and a number of kisspeptin-immunoreactive neurons. The results suggested NKB-NK3R signaling may play a dominant role in the GnRH pulse generation of KNDy neurons.
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