| Project/Area Number |
26870869
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Animal production science
Veterinary medical science
|
|---|
| Research Institution | National Institute of Health Sciences |
|---|
Principal Investigator |
ASAKURA Hiroshi 国立医薬品食品衛生研究所, 食品衛生管理部第一室, 室長 (40370936)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | Campylobacter jejuni / Chicken / microbiota / カンピロバクター / 鶏 / 菌叢変動 / マクロファージ / 盲腸菌叢 |
|---|
| Outline of Final Research Achievements |
This study examined dynamics of microbiota in chicken caecum upon C. jejuni infection. Clostridia was mostly dominant at 2 weeks-age chickens, and C. jejuni infected birds exhibited spatiotemporal increase in the yields of Faecalibacterium spp. The qPCR and FISH approaches then verified this genetic dynamics. Infiltration of Heterophiles toward caecum were significantly increased at early course of C. jejuni infection. Corroborating, chicken macrophage-like cell line HD-11 exhibited increased NO responses upon C. jejuni infection. C. jejuni also stimulated expression of several inflammatory-related genes such as TLR2/4/9.
|
