| Project/Area Number |
26870872
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
Virology
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| Research Institution | Nagasaki University |
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Principal Investigator |
MOI Meng Ling 長崎大学, 熱帯医学研究所, 准教授 (40597499)
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| Research Collaborator |
KURANE Ichiro
TAKASAKI Tomohiko
MORITA Kouichi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | デング熱 / 抗体依存感染増強 / Fc gamma receptor / dengue / ADE / flavivirus / antibody / dengue hemorrhagic fever |
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| Outline of Final Research Achievements |
In this research we aim to determine the role of Fcgamma receptor in infection-enhancement (ADE) of dengue virus (DENV). Here, we have constructed the plasmids of Fcgamma receptors (I, IIA, IIB, III), and expressed the plasmids into DENV-permissive cells to establish cell lines that stably express each of the receptors. Using these cell lines, DENV infectivity was examined using conventional plaque assay and real-time PCR assay. We then determined the ADE activity to DENV using monoclonal antibodies and patient serum samples using the Fcgamma BHK cell lines. Using these monoclonal antibodies, we determined DENV infectivity in cell lines. The ADE could be detected in cell lines that express the Fcgamma IIA and IIB receptor. Overall, we have established Fcgamma receptor expressing cell lines that are useful in DENV diagnosis and pathogenesis studies.
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