Analysis of new pathogenic bacteria as a cause substance of unknown food-borne infectious disease

• Project/Area Number: 26870873
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology); Hygiene and public health
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: ISHIHARA Tomoko 国立感染症研究所, 細菌第一部, 客員研究員 (30450555)
• Research Collaborator: SETO Kazuko
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 食中毒 / 細菌 / 病原細菌

Outline of Final Research Achievements

We analyzed 4 kinds of new pathogenic bacteria as a cause substance of unknown food-borne infectious disease. These 4 kinds of new pathogenic bacteria did not have any virulence factor for well-known food-borne infectious disease or could not be confirmed by a conventional identification test. In culture cell level, these 4 kinds of bacteria did not have the similarity of the mode of infection. Genomic analysis suggested a possibility that a close relation of these bacteria is low. These bacteria may cause the disease by a mechanism different from known pathogenic bacteria of food-borne infectious disease.
These results would contribute to investigation to determine the cause of food-borne infectious disease and to prevention of their disease.

Research Products

• [Journal Article] A Geographically Widespread Outbreak Investigation and Development of a Rapid Screening Method Using Whole Genome Sequences of Enterohemorrhagic Escherichia coli O121 (2017)
  • Author(s): Lee Ken-ichi、Morita-Ishihara Tomoko、Iyoda Sunao、Ogura Yoshitoshi、Hayashi Tetsuya、Sekizuka Tsuyoshi、Kuroda Makoto、Ohnishi Makoto、EHEC Working Group in Japan
  • Journal Title: Frontiers in Microbiology Volume: 8 Pages: 701-701
  • DOI: 10.3389/fmicb.2017.00701
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of Shiga toxin-producing <i>Escherichia coli</i> from feces of sika deer (<i>Cervus nippon</i>) in Japan using PCR binary typing analysis to evaluate their potential human pathogenicity (2017)
  • Author(s): Kabeya H, Sato S, Oda S, Kawamura M, Nagasaka M, Kuranaga M, Yokoyama E, Hirai S, Iguchi A, Ishihara T, Kuroki T, Morita-Ishihara T, Iyoda S, Terajima J, Ohnishi M, Maruyama S.
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 5 Pages: 834-841
  • DOI: 10.1292/jvms.16-0568
  • NAID: 130005635466
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular epidemiological analyses using whole genome sequencing of enterohemorrhagic Escherichia coli (2017)
  • Author(s): Lee KI, Morita-Ishihara T, Izumiya H, Iyoda S, Ohnishi M
  • Journal Title: 化学療法の領域 Volume: 33 Pages: 137-141
• [Journal Article] Secondary Shiga Toxin-Producing Escherichia coli Infection, Japan, 2010-2012. (2016)
  • Author(s): Morita-Ishihara T, Iyoda S, Iguchi A, Ohnishi M.
  • Journal Title: Emerg Infect Dis. Volume: 22(12) Issue: 12 Pages: 2181-2184
  • DOI: 10.3201/eid2212.160783
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 2015-2016年におけるEHEC広域PFGE型の発生動向 (2016)
  • Author(s): 石原朋子、伊豫田淳、泉谷秀昌、李謙一、大西真
  • Organizer: 第20回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 富山
  • Year and Date: 2016-11-10
• [Presentation] 2015年のNon-O157/O26/O111 腸管出血性大腸菌における分子疫学解析 (2016)
  • Author(s): 石原朋子，伊豫田淳，泉谷秀昌，大西真
  • Organizer: 第37回日本食品微生物学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 2016-09-15
• [Presentation] Trend of enterohemorrhagic E.coli O146 in Japan (2016)
  • Author(s): 石原朋子、伊豫田淳、寺嶋淳、泉谷秀昌、大西真、EHEC Working group
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2016-03-23
• [Presentation] Potential risk of Shiga-toxin producing E. coli (STEC) infection: investigation of STEC isolates from asymptomatic carrier in Japan (2015)
  • Author(s): Tomoko Morita-Ishihara、Sunao Iyoda、Atsushi Iguchi、Makoto Ohnishi
  • Organizer: VTEC 2015 The 9th Triennial International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia coli Infections
  • Place of Presentation: 米国、Boston
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] O157、O26、O111以外の腸管出血性大菌2014 年分離株におけるPFGE解析 (2015)
  • Author(s): 石原朋子、伊豫田淳、泉谷秀昌、大西真、EHECワーキング グループ
  • Organizer: 第19回腸管出血性大菌感染症研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-07-09
• [Presentation] 日本における健常者由来腸管出血性大腸菌の解析 (2015)
  • Author(s): 石原朋子、伊豫田淳、井口純、大西真
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 健常者糞便由来の腸管出血性大腸菌についての解析 (2014)
  • Author(s): 石原朋子、伊豫田淳、井口純、大西真
  • Organizer: 第１８回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 京都
  • Year and Date: 2014-07-15