Identification of microRNA-27b as a master regulator for breast cancer stem cell generation
| Project/Area Number |
26870877
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor therapeutics
Biological pharmacy
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
Ryou-u Takahashi 国立研究開発法人国立がん研究センター, その他部局等, 研究員 (10625510)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | がん治療 / 乳がん / microRNA / がん幹細胞 |
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| Outline of Final Research Achievements |
In the present study, we identified microRNA-27b (miR-27b) as a key regulator for the generation of cancer stem cells (CSCs). In breast caner, down-regulation of miR-27b induced the generation of side-population that shows CSC properties such as drug resistance and high tumorigenicity. MiR-27b reduced the side-population via direct repression of ectonucleotide pyrophosphatase 1 (ENPP1), which is involved in type II diabetes (T2D) development. ENPP1 induced the generation of the side-population by promoting the expression and cell surface localization of ABCG2. More importantly, a significant correlation between ENPP1 expression and breast tumor malignancy in clinical samples was observed. Overall, our study demonstrates that a T2D-associated gene plays an important role in the regulation of CSC properties and suggests that conventional chemotherapy with modulating miR-27b expression by RNA-based treatments may improve the therapeutic outcomes of breast cancer patients.
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] Establishment and characterization of an in vitro model of ovarian cancer stem-like cells with an enhanced proliferative capacity.2016
Author(s)
Ishiguro T, Sato A, Ohata H, Ikarashi Y, Takahashi R, Ochiya T, Yoshida M, Tsuda H, Onda T, Kato T, Kasamatsu T, Enomoto T, Tanaka K, Nakagama H, Okamoto K.
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Journal Title
Cancer Research
Volume: 76
Issue: 1
Pages: 150-160
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Expression of ribophorin II (RPN2) in human breast cancer2015
Author(s)
Makiko Ono, Hitoshi Tsuda, Takayuki Kobayashi, Fumitaka Takeshita, Ryou-u Takahashi, Kenji Tamura, Sadako Akashi-Tanaka, Tomoyuki Moriya, Tamio Yamasaki, Takayuki Kinoshita, Junji Yamamoto, Yasuhiro Fujiwara, and Takahiro Ochiya
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Journal Title
Pathology International
Volume: 65
Issue: 6
Pages: 301-308
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Clinical relevance and therapeutic significance of microRNA-133a expression profiles and functions in malignant osteosarcoma-initiating cells.2014
Author(s)
Fujiwara T, Katsuda T, Hagiwara K, Kosaka N, Yoshioka Y, Takahashi RU, Takeshita F, Kubota D, Kondo T, Ichikawa H, Yoshida A, Kobayashi E, Kawai A, Ozaki T, Ochiya T.
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Journal Title
Stem Cells.
Volume: 32
Issue: 4
Pages: 959-973
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 乳がんにおけるがん幹細胞形質獲得機構の解明2015
Author(s)
髙橋陵宇, 宮崎裕明, 竹下文隆, 山本雄介, 小野麻紀子, 田村 研治, 落谷孝広
Organizer
日本癌学会学術総会
Place of Presentation
愛知県 名古屋市 熱田区熱田西町1番1号 名古屋国際会議場
Year and Date
2015-10-08
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