Elucidation of phospholipid metabolism necessary for life
Project/Area Number |
26870879
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Embryonic/Neonatal medicine
General medical chemistry
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Research Institution | National Center for Global Health and Medicine |
Principal Investigator |
Yoshida Tomomi 国立研究開発法人国立国際医療研究センター, その他部局等, その他 (30610216)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 発生医学 / 生体膜リン脂質 |
Outline of Final Research Achievements |
This study demonstrated that LPCAT3, which is one of lysophospholipid acyltransferase, played a key role in biosynthesis of arachidonic acid containing phospholipids in vivo. LPCAT3-deficient mice showed a lethal phenotype within one week after birth. We also found that a triacylglycerol (TG) accumulation occurred by LPCAT3 deletion in small intestine at postnatal day 1. The study showed that an arachidonic acid containing phospholipid was important for an efficient TG transfer by an enzymatic assay in vitro. A glucose level and a TG level in blood were decreased in LPCAT3-deficient mice because enterocyte was disrupted by the accumulation. These results suggested that the dysfunction of enterocyte was one of the reasons for the neonatal lethal phenotype.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] Fatty acid remodeling by LPCAT3 enriches arachidonate in phospholipid membranes and regulates triglyceride transport.2015
Author(s)
Hashidate-Yoshida T, Harayama T, Hishikawa D, Morimoto R, Hamano F, Tokuoka SM, Eto M, Tamura-Nakano M, Yanobu-Takanashi R, Mukumoto Y, Kiyonari H, Okamura T, Kita Y, Shindou H, Shimizu T.
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Journal Title
Elife
Volume: Apr 21;4
Pages: 4-4
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Selective inhibitors of a PAF biosynthetic enzyme lysophosphatidylcholine acyltransferase 2.2014
Author(s)
Tarui M, Shindou H, Kumagai K, Morimoto R, Harayama T, Hashidate T, Kojima H, Okabe T, Nagano T, Nagase T, Shimizu T.
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Journal Title
J Lipid Res
Volume: 55
Pages: 1386-1396
Related Report
Peer Reviewed
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