| Project/Area Number |
26870924
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
Virology
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| Research Institution | National Institutes of Biomedical Innovation, Health and Nutrition |
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Principal Investigator |
Okamura Tomotaka 国立研究開発法人医薬基盤・健康・栄養研究所, 霊長類医科学研究センター, 研究員 (20549703)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 免疫不全ウイルス / 細胞性免疫 / アジュバント / エイズウイルス / 弱毒エイズワクチン / サル動物モデル / エフェクターメモリーT細胞 |
|---|
| Outline of Final Research Achievements |
The Ag85B can induce strong Th1-type immune responses in mice as an adjuvant. We previously reported that the cDNA of Ag85B was inserted into simian-human immunodeficiency virus (SHIV) eliminated nef gene of SHIV (SHIV-Ag85B). In the present study, immune responses in cynomolgus monkeys infected with the SHIV-Ag85B were analyzed, and also examined immune responses in those monkeys after infection with pathogenic SHIV (SHIV89.6P). The SHIV-Ag85B infected monkeys elicited Gag-specific T cell responses. When monkeys infected with SHIV-Ag85B were challenged with pathogenic SHIV89.6P, those monkeys showed protective effects for the intravenous challenge of pathogenic SHIV89.6P. The monkeys pre-infected with SHIV-Ag85B showed the enhancement of Gag-specific IFN-γ producing T cells after SHIV89.6P intravenously injection. These results suggest that the SHIV-Ag85B elicited strong immune responses against SHIV and could provide protective effects to pathogenic SHIV89.6P infection.
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