| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Extracts from subcutaneous tissues of the deep sea fish have activity to induce spheroid formation on cultured mammalian cells. Candidate protein A isolated from the deep sea fish extract is a secreted protein that is conserved in a wide range of species, and there has been no report that has activity of spheroid formation. Spheroidization activity of A was confirmed by fish genes, but it could not be confirmed with mammalian homologous genes, suggesting that the induction of spheroid formation by A is a phenomenon found only in A of fish. In order to search for the target protein for A to promote cell adhesion, we performed pull down assay of A targeted to cell lysate. We found B, a protein localized in the adherence junction of the cell and stabilizes cell-cell adhesion. We analyzed cell polarity in spheroid made with deep-sea fish extract. It is suggested that the spheroids formed by the deep sea extract are not mere cell clusters but aggregates according to cell polarity.
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