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Elucidation of the molecular basis of spheroid formation by deep-sea extract

Research Project

Project/Area Number 26870930
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Cell biology
Research Institution地方独立行政法人鳥取県産業技術センター

Principal Investigator

Yuko Nakamura-Sugimoto  地方独立行政法人鳥取県産業技術センター, バイオ技術科, 研究員 (10443665)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords細胞間相互作用 / 細胞外マトリックス
Outline of Final Research Achievements

Extracts from subcutaneous tissues of the deep sea fish have activity to induce spheroid formation on cultured mammalian cells. Candidate protein A isolated from the deep sea fish extract is a secreted protein that is conserved in a wide range of species, and there has been no report that has activity of spheroid formation. Spheroidization activity of A was confirmed by fish genes, but it could not be confirmed with mammalian homologous genes, suggesting that the induction of spheroid formation by A is a phenomenon found only in A of fish. In order to search for the target protein for A to promote cell adhesion, we performed pull down assay of A targeted to cell lysate. We found B, a protein localized in the adherence junction of the cell and stabilizes cell-cell adhesion. We analyzed cell polarity in spheroid made with deep-sea fish extract. It is suggested that the spheroids formed by the deep sea extract are not mere cell clusters but aggregates according to cell polarity.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2017 2016

All Journal Article (1 results) Presentation (2 results)

  • [Journal Article] 未利用海洋資源のバイオ素材への応用2017

    • Author(s)
      中村(杉本)優子
    • Journal Title

      Food Sytle 21

      Volume: 21 Pages: 34-37

    • Related Report
      2016 Annual Research Report
  • [Presentation] 深海魚抽出物によるスフェロイド形成の分子基盤の解明2016

    • Author(s)
      中村(杉本)優子
    • Organizer
      第39回日本分子生物学会大会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生体由来素材によるスフェロイド培養法の開発2016

    • Author(s)
      中村(杉本)優子
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      宮城県仙台市
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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