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The role of the genetic structure of host populations on the evolution of an emerging viral pathogen

Research Project

Project/Area Number 26881007
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Environmental impact assessment
Research InstitutionOsaka Ohtani University

Principal Investigator

UCHII Kimiko  大阪大谷大学, 薬学部, 助教 (90469619)

Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords新興感染症 / コイヘルペスウイルス / 環境DNA
Outline of Final Research Achievements

To examine the interactions between the evolution of an emerging Cyprinid herpesvirus 3 (CyHV3) and the population genetic structures of host common carp (Cyprinus carpio), I developed an environmental DNA-based method to evaluate the carp population genetic structures and performed a preliminary survey of CyHV3 genetic variations. The environmental DNA method successfully provided quantitative estimates for the proportion of genotypes in wild carp populations. CyHV3 collected from natural water bodies several years after its introduction had multiple genotypes, even though a single genotype was initially spread in Japan, suggesting that CyHV3 evolved after its introduction.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (7 results)

All 2016 2015

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (4 results) Book (1 results)

  • [Journal Article] A novel environmental DNA approach to quantify the cryptic invasion of non-native genotypes2016

    • Author(s)
      Uchii, K., Doi, H. and Minamoto, T.
    • Journal Title

      Molecular Ecology Resources

      Volume: 16 Issue: 2 Pages: 415-422

    • DOI

      10.1111/1755-0998.12460

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Use of droplet digital PCR for estimation of fish abundance and biomass in environmental DNA surveys2015

    • Author(s)
      DOI Hiedeyuki ; UCHII Kimiko ; TAKAHARA Teruhiko ; MATSUHASHI Saeko ; YAMANAKA Hiroki ; MINAMOTO Toshifumi
    • Journal Title

      PLOS ONE

      Volume: 10 Issue: 3 Pages: e0122763-e0122763

    • DOI

      10.1371/journal.pone.0122763

    • NAID

      120006220783

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] SNPの定量的解析による遺伝子型頻度の推定2016

    • Author(s)
      内井喜美子, 土居秀幸, 源利文, 山中裕樹
    • Organizer
      第63回日本生態学会
    • Place of Presentation
      仙台国際センター(仙台)
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] コイヘルペスウイルスの野外における増殖/潜伏サイクル2015

    • Author(s)
      酒井涼, 内井喜美子, 谷佳津治
    • Organizer
      第65回日本薬学会近畿支部総会・大会
    • Place of Presentation
      大阪大谷大学(富田林)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 環境DNA分析によるコイの季節移動の推定2015

    • Author(s)
      内井喜美子, 土居秀幸, 山中裕樹, 源利文
    • Organizer
      日本陸水学会第80回大会
    • Place of Presentation
      北海道大学(函館)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 環境DNA手法を用いた在来および外来コイの生息地利用の推定2015

    • Author(s)
      内井喜美子, 土居秀幸, 山中裕樹, 源利文
    • Organizer
      第62回日本生態学会大会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2015-03-19
    • Related Report
      2014 Annual Research Report
  • [Book] コイヘルペスウイルス. 川端善一郎他 (編) "感染症の生態学" pp. 241-2532016

    • Author(s)
      内井喜美子, 川端善一郎
    • Total Pages
      356
    • Publisher
      共立出版
    • Related Report
      2015 Annual Research Report

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Published: 2014-09-09   Modified: 2017-05-10  

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