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Investigation of reactive species generated by low temperature plasma and fundamental research for plasma medicine

Research Project

Project/Area Number 26886009
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Plasma electronics
Research InstitutionKobe University

Principal Investigator

Takamatsu Toshihiro  神戸大学, 医学(系)研究科(研究院), 研究員 (10734949)

Research Collaborator Azuma Takeshi  神戸大学, 大学院医学研究科消化器内科学分野, 教授 (60221040)
Okino Akitoshi  東京工業大学, 未来産業技術研究所, 准教授 (60262276)
Ito Norihiko  鳥取大学, 農学部附属動物医療センター, 准教授 (80264654)
Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords大気圧低温プラズマ / プラズマ医療 / 活性種 / 殺菌
Outline of Final Research Achievements

Using developed temperature-controllable multi-gas plasma, generation of reactive species, bacterial inactivation, optimization of plasma source and effect on living body were investigated for medical application. As the results, it is found that nitrogen plasma and carbon dioxide plasma, which generate a large amount of OH radical and singlet oxygen, have high bacterial inactivation effect. We suggest plasma bubble water, and the bacterial inactivation effect was confirmed. Using 3D printer, we succeeded to develop new plasma source, which can be inserted in endoscope. And the treatments with these plasma sources were free from damage to living body.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (32 results)

All 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (27 results) (of which Int'l Joint Research: 3 results) Book (1 results)

  • [Journal Article] Atmospheric nonequilibrium mini-plasma jet created by a 3D printer2015

    • Author(s)
      T. Takamatsu, H. Kawano, H. Miyahara, T. Azuma and A. Okino
    • Journal Title

      AIP Advances

      Volume: 5 Issue: 7 Pages: 077184-077184

    • DOI

      10.1063/1.4928034

    • NAID

      120005650143

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Microbial Inactivation in the Liquid Phase Induced by Multigas Plasma Jet2015

    • Author(s)
      T. Takamatsu, K. Uehara, Y. Sasaki, M. Hidekazu, Y. Matsumura, A. Iwasawa, N. Ito, M. Kohno, T. Azuma and A. Okino
    • Journal Title

      PLOS ONE

      Volume: 10 Issue: 7 Pages: e0132381-e0132381

    • DOI

      10.1371/journal.pone.0132381

    • NAID

      120005650145

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Temperature Controllable Atmospheric Plasma Source2015

    • Author(s)
      T. Oshita, H. Kawano, T. Takamatsu, H. Miyahara and A. Okino
    • Journal Title

      IEEE Trans on Plasma Science

      Volume: 43 Issue: 6 Pages: 1987-1992

    • DOI

      10.1109/tps.2015.2428696

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigation of reactive species using various gas plasmas2014

    • Author(s)
      Toshihiro Takamatsu, Kodai Uehara, Yota Sasaki, Hidekazu Miyahara, Yuriko Matsumura, Atsuo Iwasawa, Norihiko Ito, Takeshi Azuma, Masahiro Kohno and Akitoshi Okino
    • Journal Title

      RSC Advances

      Volume: 4 Issue: 75 Pages: 39901-39905

    • DOI

      10.1039/c4ra05936k

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Development of atmospheric non-thermal mini-plasma jet created by a 3D printer2015

    • Author(s)
      T. Takamatsu, H. Kawano, H. Miyahara, T. Azuma and A. Okino
    • Organizer
      第25回日本MRS年次大会
    • Place of Presentation
      神奈川
    • Year and Date
      2015-12-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] ガス種及びガス温度がプラズマバブル水中の活性種に与える影響2015

    • Author(s)
      小林智裕, 菅原修馬, 川野浩明, 高松利寛, 松村有里子, 宮原秀一, 岩澤篤郎, 東 健, 沖野晃俊
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 各種ガスプラズマによる水中殺菌と細菌の電子顕微鏡観察2015

    • Author(s)
      堂山英之, 川野浩明, 小林智裕, 高松利寛, 松村有里子, 宮原秀一, 岩澤篤郎, 東健, 沖野晃俊
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 様々なガス種のプラズマによる殺菌効果と細菌の電子顕微鏡観察2015

    • Author(s)
      高松利寛,佐々木洋太,松村有里子,岩澤篤郎,宮原秀一,河野雅弘,東健,沖野晃俊
    • Organizer
      日本防菌防黴学会 第42回年次大会
    • Place of Presentation
      大阪
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヘルスケア・医療分野への応用に向けた大気圧低温プラズマの開発2015

    • Author(s)
      高松利寛
    • Organizer
      第1回先端錯体工学研究会ミニシンポジウム
    • Place of Presentation
      神奈川
    • Year and Date
      2015-07-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Influence of plasma gas temperature on inactivation effect to various bacteria2015

    • Author(s)
      H. Kawano, T. Oshita, T. Takamatsu, Y. Matsumura, H. Miyahara, A. Iwasawa, T. Azuma and A. Okino
    • Organizer
      ISPC22
    • Place of Presentation
      Belgium
    • Year and Date
      2015-07-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 各種ガスプラズマによる水中殺菌とESRおよび吸光光度法による活性種測定2015

    • Author(s)
      川野浩明, 渡辺洋輔, 佐々木洋太, 高松利寛, 松村有里子, 宮原秀一, 岩澤篤郎, 東健, 沖野晃俊
    • Organizer
      平成27年度 日本分光学会年次講演会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 殺菌・洗浄を目的としたプラズマバブル水の活性種測定2015

    • Author(s)
      小林智裕, 渡辺洋輔, 大下貴也, 高松利寛, 松原裕樹, 大島悟, 神谷哲, 松村有里子, 宮原秀一, 岩澤篤郎, 東 健, 沖野晃俊
    • Organizer
      平成27年度 日本分光学会年次講演会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Measurement of sterilization ability and reactive species of various plasma bubbled-up water2015

    • Author(s)
      Tomohiro Kobayashii, Yosuke Watanabe, Takaya Oshita, Toshihiro Takamatsu, Hiroki Matsubara, Satoru Oshima, Tetsu Kamiya, Yuriko Matsumura, hidekadzu miyahara, Atsuo Iwasawa, Takeshi Azuma, Akitoshi Okino
    • Organizer
      The 42th IEEE International Coference on Plasma Science
    • Place of Presentation
      Turkey
    • Year and Date
      2015-05-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation of Bacterial Inactivation by Various Gas Plasmas and Electron Microscopic Observation of Treated Bacteria2015

    • Author(s)
      Toshihiro Takamatsu, Tomohiro Kobayashi, Hiroaki Kawano, Yota Sasaki, Yosuke Watanabe, Yuriko Matsumura, Hidekazu Miyahara, Atsuo Iwasawa, Takeshi Azuma and Akitoshi Okino
    • Organizer
      The 42th IEEE International Coference on Plasma Science
    • Place of Presentation
      Turkey
    • Year and Date
      2015-05-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] プラズマバブル水の活性種測定と細菌不活化効果の調査2015

    • Author(s)
      小林智裕, 渡辺洋輔, 大下貴也, 高松利寛*, 松原裕樹, 大島悟, 神谷晢, 松村有里子, 宮原秀一, 岩澤篤郎, 東健, 沖野晃俊
    • Organizer
      平成26年度 第4回 電気学会東京支部神奈川支部研究会
    • Place of Presentation
      神奈川
    • Year and Date
      2015-02-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] マルチガスプラズマジェットを用いた殺菌効果に寄与する活性種の調査2014

    • Author(s)
      高松利寛*,佐々木洋太,宮原秀一,岩澤篤郎,松村有里子,河野雅弘,東健,沖野晃俊
    • Organizer
      第24回 日本MRS年次大会
    • Place of Presentation
      神奈川
    • Year and Date
      2014-12-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Water sterilization and clarification by multi-gas plasma-bubbling2014

    • Author(s)
      渡辺 洋輔, 大下 貴也, 高松 利寛*, 小林 智裕, 松原裕樹,大島悟,神谷晢, 松村 有里子, 宮原 秀一, 岩澤 篤郎, 東 健, 沖野 晃俊
    • Organizer
      第24回 日本MRS年次大会
    • Place of Presentation
      神奈川
    • Year and Date
      2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 様々なガスを用いたプラズマバブル水の殺菌効果調査2014

    • Author(s)
      小林智裕,渡辺洋輔,大下貴也,高松利寛*,松原裕樹,大島悟,神谷晢,松村有里子,宮原秀一,岩澤篤郎,東健,沖野晃俊
    • Organizer
      第24回 日本MRS年次大会
    • Place of Presentation
      神奈川
    • Year and Date
      2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Influence of plasma gas species on bacterial inactivation and reactive species production by plasma-bubbling2014

    • Author(s)
      Toshihiro Takamatsu*, Yosuke Watanabe, Hidekazu Miyahara, Yuriko Matsumura, Atsuo Iwasawa, Masahiro Kohno, Akitoshi Okino
    • Organizer
      Asia-Pacific EPR/ESR Symposium 2014
    • Place of Presentation
      奈良
    • Year and Date
      2014-11-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Investigation of Reactive Species Generated by Various Gas Plasmas using ESR Spin Trapping Method2014

    • Author(s)
      Yuriko Matsumura, Toshihiro Takamatsu*, Kodai Uehara, Yota Sasaki, Hidekazu Miyahara, Atsuo Iwasawa, Masahiro Kohno, Akitoshi Okino
    • Organizer
      Asia-Pacific EPR/ESR Symposium 2014
    • Place of Presentation
      奈良
    • Year and Date
      2014-11-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Investigation of Sterilization Effect by Various Gas Plasmas and Electron Microscopic Observation of Bacteria2014

    • Author(s)
      Yota Sasaki, Toshihiro Takamatsu*, Hidekazu Miyahara, Yuriko Matsumura, Atsuo Iwasawa, Masahiro Kohno, Keiko Ikeda, Akitoshi Okino
    • Organizer
      56th Annual Meeting of the APS Division of Plasma Physics
    • Place of Presentation
      New Orleans, US
    • Year and Date
      2014-10-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 殺菌効果および活性種生成量のプラズマガス温度依存性の調査2014

    • Author(s)
      川野浩明, 大下貴也, 高松利寛*, 松村有理子, 宮原秀一, 岩澤篤郎, 東健, 沖野晃俊
    • Organizer
      電気学会 プラズマ/パルスパワー/放電 合同研究会
    • Place of Presentation
      大分
    • Year and Date
      2014-10-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Investigation of ROS Production Process using Multi-Gas Plasma2014

    • Author(s)
      Toshihiro Takamatsu*, Yota Sasaki, Hidekazu Miyahara, Yuriko Matsumura, Atsuo Iwasawa, Masahiro Kohno, Takeshi Azuma*, Akitoshi Okino.
    • Organizer
      THE 21ST INTERNATIONAL SPACC SYMPOSIUM
    • Place of Presentation
      東京
    • Year and Date
      2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 各種ガスのプラズマを導入した水による一般細菌の殺菌効果検証2014

    • Author(s)
      小林智裕, 渡辺洋輔, 大下貴也, 高松利寛*, 松村有里子, 宮原秀一, 岩澤篤郎, 東健, 沖野晃俊
    • Organizer
      日本防菌防黴学会 第41回年次大会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 各種ガスプラズマ照射で生成される液中活性酸素種のESR測定2014

    • Author(s)
      佐々木洋太, 高松利寛*, 上原広大, 大下貴也, 宮原秀一, 岩澤篤郎, 松村有里子, 東健, 河野雅弘, 沖野晃俊
    • Organizer
      日本防菌防黴学会 第41回年次大会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] プラズマガス温度が殺菌効果および活性種生成量に与える影響2014

    • Author(s)
      川野浩明, 大下貴也, 高松利寛*, 松村有理子, 宮原秀一, 岩澤篤郎, 東健*, 沖野晃俊
    • Organizer
      日本防菌防黴学会 第41回年次大会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] プラズマバブリングによる活性酸素種の水中導入と各種細菌の不活化2014

    • Author(s)
      渡辺洋輔, 大下貴也, 高松利寛*, 松村有里子, 宮原秀一, 岩澤篤郎, 東健*, 沖野晃俊
    • Organizer
      日本防菌防黴学会 第41回年次大会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 様々なガス種の低温プラズマによる殺菌及び毒素の分解効果の検証2014

    • Author(s)
      高松利寛*, 上原広大, 佐々木洋太, 松村有里子, 岩澤篤郎,宮原秀一,河野雅弘, 東健, 沖野晃俊
    • Organizer
      日本防菌防黴学会 第41回年次大会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Bacterial Inactivation Using Ultrafine Bubble Water Produced by Atmospheric Multi-gas Plasma2014

    • Author(s)
      Takaya Oshita, Yosuke Watanabe, Toshihiro Takamatsu*, Masakazu Kashiwa, Hidekazu Miyahara, Toshihiro Fujita, Akitoshi Okino
    • Organizer
      Particulate System Analysis 2014
    • Place of Presentation
      Manchester, UK
    • Year and Date
      2014-09-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Investigation of Bacterial Inactivation by Reactive Species using Various Gas Plasmas2014

    • Author(s)
      Toshihiro Takamatsu*, Kodai Uehara, Yota Sasaki, Hidekazu Miyahara, Yuriko Matsumura, Atsuo Iwasawa, Takeshi Azuma, Masahiro Kohno, Akitoshi Okino.
    • Organizer
      5th Euro-Asian Pulsed Power Conference
    • Place of Presentation
      熊本
    • Year and Date
      2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Influence of Plasma Gas Temperature on Sterilizing Effect and Active-Speacies Generation2014

    • Author(s)
      Hiroaki Kawano, Takaya Oshita, Toshihiro Takamatsu*, Yuriko Matsumura, Hidekazu Miyahara, Atsuo Iwasawa, Takeshi Azuma, Akitoshi Okino.
    • Organizer
      5th Euro-Asian Pulsed Power Conference
    • Place of Presentation
      熊本
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Book] Gas Plasma Sterilization in Microbiology: Theory, Applications, Pitfalls and New Perspectives2016

    • Author(s)
      T. Takamatsu, H. Miyahara, T. Azuma and A. Okino
    • Total Pages
      158
    • Publisher
      Caister Academic Press
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-09-09   Modified: 2017-05-10  

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