Development of an effective folding procedure for SS-containing proteins by using a micro flow chemistry
Project/Area Number |
26888016
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Bio-related chemistry
|
Research Institution | Tokai University |
Principal Investigator |
Arai Kenta 東海大学, 理学部, 助教 (60728062)
|
Project Period (FY) |
2014-08-29 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | フォールディング / マイクロリアクター / ジスルフィド / フローケミストリー / インスリン / リゾチーム / ジスルフィド結合 / タンパク質 |
Outline of Final Research Achievements |
A chemically or biologically synthesized polypeptide needs to fold into the bioactive native form through random disulfide (SS) formation and SS isomerization. In this research, development of a facile methodology, which allows any scientists in various fields to achieve an effective folding, was attempted only by using a micro flow reactor (MR), which enables a rapid reaction due to the very short diffusion distances, without special organic reagents. When the each solution of reduced lysozyme as a model protein and glutathione was flowed into the MR at the same time, the bioactive lysozyme was gradually recovered. It is worth noting that the refolding velocity under a flow condition was faster than that under a batch condition. Similar folding behavior was observed for the folding of double-chain insulin coupled with inter-chain SS formation. In conclusion, the micro flow technology has enabled the effective oxidative folding reaction without a complicating methodology.
|
Report
(3 results)
Research Products
(9 results)
-
-
-
-
[Journal Article] Fatty acid conjugates of water-soluble trans-3,4-dihydroxy-1-selenolane. Effect of an alkyl chain length on the antioxidant capacity2015
Author(s)
Michio Iwaoka, Nobukazu Sano, Ying-Yin Lin, Arisa Katakura, Masato Noguchi, Kohei Takahashi, Fumio Kumakura, Kenta Arai, Beena G. Singh, Amit Kunwar, K. Indira Priyadarsini
-
Journal Title
Related Report
Peer Reviewed
-
-
-
-
-