| Project/Area Number |
26888016
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Bio-related chemistry
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| Research Institution | Tokai University |
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Principal Investigator |
Arai Kenta 東海大学, 理学部, 助教 (60728062)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | フォールディング / マイクロリアクター / ジスルフィド / フローケミストリー / インスリン / リゾチーム / ジスルフィド結合 / タンパク質 |
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| Outline of Final Research Achievements |
A chemically or biologically synthesized polypeptide needs to fold into the bioactive native form through random disulfide (SS) formation and SS isomerization. In this research, development of a facile methodology, which allows any scientists in various fields to achieve an effective folding, was attempted only by using a micro flow reactor (MR), which enables a rapid reaction due to the very short diffusion distances, without special organic reagents. When the each solution of reduced lysozyme as a model protein and glutathione was flowed into the MR at the same time, the bioactive lysozyme was gradually recovered. It is worth noting that the refolding velocity under a flow condition was faster than that under a batch condition. Similar folding behavior was observed for the folding of double-chain insulin coupled with inter-chain SS formation. In conclusion, the micro flow technology has enabled the effective oxidative folding reaction without a complicating methodology.
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