| Project/Area Number |
26889028
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Jeong Hee-Jin 東京工業大学, 資源化学研究所, 助教 (70737981)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | クエンチボディー / クローディン / 免疫センサー / 細胞イメージング / 蛋白質工学 / 抗体工学 / 蛍光解除 / 生細胞イメージング |
|---|
| Outline of Final Research Achievements |
In this study, we successfully detected claudins which are abnormally expressed on cancer cells by Quenchbody(Q-body)-based simple and convenient novel immunoassay method. We constructed Q-body using anti-claudin antibody gene and measured the fluorescent intensity in the present of purified claudin. As a result, the intensity was claudin-concentration dependently increased and showed the maximum 3.1-fold response. Moreover, when we added the Q-body into the claudin-expressed cells, it showed significant fluorescent, indicating the effort of this Q-body for imaging the over-expressed claudins.
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