| Project/Area Number |
26890001
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical genome science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Onozawa Masahiro 北海道大学, 医学(系)研究科(研究院), 助教 (70455632)
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| Research Collaborator |
APLAN Peter NIH/NCI, Genetics branch, Senior investigator
MIYASHITA Naohiro
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | テロメラーゼ / DNA損傷 / DNA修復 / 遺伝多型 / CRISPR / テロメア / 鋳型配列挿入多型 |
|---|
| Outline of Final Research Achievements |
Telomerase has various biological functions other than elongation of telomere repeat at chromosomal end. Although previous study suspected that telomerase play role for DNA repair mechanisms, there was no direct evidence. In this study, we showed induced DNA double strand breaks could be repaired by insertion of telomere repeat (TTAGGG)n in cell line. We also revealed that telomere repeat insertion is one of the human genome polymorphisms. Interstitial telomeric sequences are considered to be trace of DNA break repair events, and this mechanisms were evolutionally conserved mechanisms to repair DNA breakage.
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