| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We previously reported male-to-female sex reversal in H3K9 demethylase Jmjd1a knockout mice. However the involvement of other H3K9me-regulating enzymes in sex determination was unknown. We compare the consequence of combined loss of Jmjd1a and GLP on the sex-reversal phenotype. Loss of even a single GLP allele could rescue the sex-reversal phenotype in Jmjd1a-deficient background, which is accompanied by increase of Sry expression, indicating the functional antagonism between Jmjd1a and GLP. On the other hand, loss of a Jmjd1b single could allele accelerate the sex-reversal in Jmjd1a-deficient mice, indicating the overlapping role of Jmjd1b in mouse sex determination. These results suggest the fine tuning of H3K9 methylation level by methylases and demethylases is vital for mouse sex determination.
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