Experimental evolution of an RNA enzyme using droplet-based microfluidics
Project/Area Number |
26891009
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Evolutionary biology
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Research Institution | University of Toyama |
Principal Investigator |
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Research Collaborator |
Griffiths Andrew D. ESPCI ParisTech, 教授
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Project Period (FY) |
2014-08-29 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 人工進化 / RNA酵素 / マイクロ流体システム / エマルジョン / 進化生物学 / 進化分子工学 / RNA / リボザイム / 人工細胞 |
Outline of Final Research Achievements |
In this study, we focused on developing a compartmentalized ribozyme evolution system using microfluidic technology to pursue potential of catalytic RNAs. It is composed of two parts; “compartmentalized ribozyme expression system” and “droplet-based microfluidics”. First, we established the former by optimizing components of an RNA expression system we have previously developed. Second, the latter, an original system which is capable of multi-color fluorescence-based analysis and sorting of droplets, was constructed. Currently, InDrop evolution experiment of ribozymes is undergoing by using the system described above. The drop microfluidic system and devices developed in this study will contribute the basic technology for further artificial RNA evolution experiments.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Mutational analysis of structural elements in a class-I cyclic di-GMP riboswitch to elucidate its regulatory mechanism.2016
Author(s)
Inuzuka, S., Nishimura, K., Kakizawa, H., Fujita, Y., Furuta, H., Matsumura, S., Ikawa, Y.
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Journal Title
The Journal of Biochemistry
Volume: 印刷中
Issue: 3
Pages: 153-162
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Experimental RNA evolution in droplets: How cellularity contributes the early RNA world evolution2015
Author(s)
Matsumura, S., Coldren, F., Kun, A., Nghe, P., Jossinet, F., Szathmary, E., Griffiths, A., Ryckelynck, M.
Organizer
Pacifichem 2015, The International Chemical Congress of Pacific Basin Societies 2015
Place of Presentation
Hawaii Convention Center(Honolulu, Hawaii, USA)
Year and Date
2015-12-16
Related Report
Int'l Joint Research
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[Presentation] 細胞構造と群淘汰がもたらす遺伝情報の進化的安定性と多様性2014
Author(s)
松村 茂祥, Coldren, F. M., Kun, A., Nghe, P., Jossinet, F., Szathmary, E., Griffiths, A. D., Ryckelynck, M.
Organizer
第37回日本分子生物学会年会
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Year and Date
2014-11-25
Related Report
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[Presentation] Evolutionary stability and diversity of genetic information established by cellularity and cell-based selection2014
Author(s)
Matsumura, S., Coldren, F., Kun, A., Nghe, P., Jossinet, F., Szathmary, E., Griffiths, A., Ryckelynck, M.
Organizer
ISNAC 2014, The 41st International Symposium on Nucleic Acids Chemistry
Place of Presentation
北九州国際会議場(福岡県北九州市)
Year and Date
2014-11-06
Related Report
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[Presentation] Empirical investigation of importance of cellularity in the RNA world evolution using droplet-based microfluidics2014
Author(s)
Matsumura, S., Coldren, F., Kun, A., Nghe, P., Jossinet, F., Szathmary, E., Griffiths, A., Ryckelynck, M.
Organizer
DNA20, The 20th International Conference on DNA Computing and Molecular Programming
Place of Presentation
京都大学医学部芝蘭会館(京都府京都市)
Year and Date
2014-09-25
Related Report
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