Visualization of the phosphatidylinositol 4,5-bisphosphate and dynamics of the Ca2+-binding protein during root hair elongation in Arabidopsis

• Project/Area Number: 26891013
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: Kato Mariko 京都大学, 化学研究所, 助教 (90736646)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 植物細胞形態形成 / 細胞内シグナル伝達 / 植物 / シグナル伝達 / 脂質

Outline of Final Research Achievements

Root hair is a tubular-shaped cell that emerges from a specific root epidermal cell and contributes to water absorption, nutrient uptake and root anchoring. As well as these important functions, root hair has been intensively studied with regard to its characteristic morphogenesis. It has been reported that multiple signals mediated by calcium, phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), reactive oxygen species and Rho-related GTPases are esssential for the root hair tip growth. Of these, PI(4,5)P2 is thought to promote root hair elongation. However, molecular mechanisms that control PI(4,5)P2 signal during tip growth is poorly understood.
Here, I visualized the dynamics of Arabidopsis PI(4,5)P2/calcium-binding protein PCaP2 and PI(4,5)P2 signal using a live-cell imaging technique. I found that PCaP2 is involved in spatio-temporal localization of PI(4,5)P2 signal on plasma membrane to control root hair tip growth.

Research Products

• [Journal Article] A novel-type phosphatidylinositol phosphate-interactive, Ca-binding protein PCaP1 in Arabidopsis thaliana: stable association with plasma membrane and partial involvement in stomata closure. (2016)
  • Author(s): Nagata, C., Miwa, C., Tanaka, N., Kato, M., Suito, M., Tsuchihira, A., Sato, Y., Segami, S. and Maeshima, M.
  • Journal Title: Journal of Plant Research Volume: In press Issue: 3 Pages: 539-550
  • DOI: 10.1007/s10265-016-0787-2
  • NAID: 120005868252
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] GLABRA2 directly suppresses basic helix-loop-helix transcription factor genes with diverse functions in root hair development. (2015)
  • Author(s): Lin, Q., Ohashi, Y., Kato, M., Tsuge, T., Gu ,H., Qu, L.-J., and Aoyama, T.
  • Journal Title: Plant Cell Volume: 27 Pages: 2894-2906
  • DOI: 10.1105/tpc.15.00607
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses. (2014)
  • Author(s): Tanaka, N., Kato, M., Tomioka, R., Kurata, R., Fukao, Y., Aoyama, T., and Maeshima, M.
  • Journal Title: Journal of Experimental Botany Volume: 65 Issue: 6 Pages: 1497-1512
  • DOI: 10.1093/jxb/eru014
  • Peer Reviewed / Open Access
• [Presentation] Cell Biological Function of Arabidopsis PLDζ2 (2016)
  • Author(s): Shimamura Ryota, Taniguchi Y. Yukimi, Kato Mariko, Tsuge Tomohiko, Aoyama Takashi
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手
  • Year and Date: 2016-03-18
• [Presentation] Regulation Roles of PIP5K Genes in Plant Cell Morphogenesis and Their Redundancy (2016)
  • Author(s): Watari Machiko, Blanc-Mathieu Romain, Kato Mariko, Tsuge Tomohiko, Ogata Hiroyuki, Aoyama Takashi,
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手
  • Year and Date: 2016-03-18
• [Presentation] シロイヌナズナ根毛におけるCa2+結合タンパク質PCaP2とホスファチジルイノシトール4,5-二リン酸の細胞内動態 (2015)
  • Author(s): 加藤真理子、柘植知彦、前島正義、青山卓史
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学、東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] PIP5K3と他のタイプB PIP5K遺伝子との機能重複に関する解析 (2015)
  • Author(s): 齊藤涼、和田悠貴香、加藤真理子、柘植知彦、青山卓史
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学、東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 側方根冠におけるシロイヌナズナPLDζ2の役割 (2015)
  • Author(s): 島村亮太、谷口（山本）幸美、谷口雅俊、加藤真理子、柘植知彦、林謙一郎、青山卓史
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学、東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 根毛伸長時におけるシロイヌナズナCa2+結合タンパク質PCaP2とホスファチジルイノシトール4,5-二リン酸の細胞内動態 (2014)
  • Author(s): 加藤真理子、柘植知彦、前島正義、青山卓史
  • Organizer: 第27回植物脂質シンポジウム
  • Place of Presentation: 静岡市産学交流センター、静岡
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] シロイヌナズナの根毛伸長におけるPIP5K遺伝子の役割 (2014)
  • Author(s): 和田悠貴香、草野博彰、亘真智子、齊藤涼、加藤真理子、柘植知彦、青山卓史
  • Organizer: 第27回植物脂質シンポジウム
  • Place of Presentation: 静岡市産学交流センター、静岡
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] シロイヌナズナの側方根冠におけるPLDζ2-PAシグナルの役割 (2014)
  • Author(s): 島村亮太、谷口（山本）幸美、谷口雅俊、加藤真理子、柘植知彦、青山卓史
  • Organizer: 第27回植物脂質シンポジウム
  • Place of Presentation: 静岡市産学交流センター、静岡
  • Year and Date: 2014-11-28 – 2014-11-29
• [Book] Plant Cell Wall Patterning and Cell Shape (Ed, Hiroo Fukuda) (2014)
  • Author(s): Hiroaki Kusano, Rumi Tominaga, Takuji Wada, Mariko Kato and Takashi Aoyama.
  • Total Pages: 432
  • Publisher: Wiley-Blackwell