NMR analysis of the structural flexibility of membrane proteins
| Project/Area Number |
26891016
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
Ozawa Kiyoshi 大阪大学, たんぱく質研究所, 研究員 (20251770)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | NMR / ナノディスク / 無細胞蛋白質合成系 / 膜蛋白質 / 蛋白質標識 |
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| Outline of Final Research Achievements |
pHtrII(1-159) protein is a bacterial phototaxis transducer membrane protein from Natronomonas pharaonis and functions as a cognate transducer to the pharaonis phoborhodpsin. The full length of this transducer has yet to be obtained owing mainly to the difficulties in obtaining this complex in a stable form that eases its structural and functional studies.
In this study, I attempted the use of the phospholipid bilayer nanodisc for studying the structure of pHtrII(1-159) in solution NMR. Cell-free expressed 15N, 2H-labelled pHtrII (1-159) transducer was purified, and it was successfully reconstituted into a nanodisc. Consequently the NMR analysis of the pHtrII (1-159) transducer in a nanodisc provides important information on structural changes between pH6.5 and pH7.0, and between with and without nanodiscs.
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Report
(3 results)
Research Products
(3 results)
